8EXA

ISDra2 TnpB in complex with reRNA and cognate DNA, conformation 1 (RuvC domain resolved)

8EXA の概要

• エントリーDOI: 10.2210/pdb8exa/pdb
• 関連するPDBエントリー: 8BF8 8EX9
• EMDBエントリー: 16016 28655 28656
• 分子名称: RNA-guided DNA endonuclease TnpB, RNA (150-MER), DNA (43-MER), ... (4 entities in total)
• 機能のキーワード: transposon, tnpb, ruvc domain, cas12, is200/is605, rerna, rna binding protein
• 由来する生物種: Deinococcus radiodurans R1; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 121424.93

構造登録者

Sasnauskas, G.,Tamulaitiene, G.,Carabias, A.,Karvelis, T.,Druteika, G.,Silanskas, A.,Montoya, G.,Venclovas, C.,Kazlauskas, D.,Siksnys, V. (登録日: 2022-10-25, 公開日: 2023-04-05, 最終更新日: 2024-06-19)

主引用文献

Sasnauskas, G.,Tamulaitiene, G.,Druteika, G.,Carabias, A.,Silanskas, A.,Kazlauskas, D.,Venclovas, C.,Montoya, G.,Karvelis, T.,Siksnys, V.
TnpB structure reveals minimal functional core of Cas12 nuclease family.
Nature, 616:384-389, 2023

PubMed Abstract: The widespread TnpB proteins of IS200/IS605 transposon family have recently emerged as the smallest RNA-guided nucleases capable of targeted genome editing in eukaryotic cells. Bioinformatic analysis identified TnpB proteins as the likely predecessors of Cas12 nucleases, which along with Cas9 are widely used for targeted genome manipulation. Whereas Cas12 family nucleases are well characterized both biochemically and structurally, the molecular mechanism of TnpB remains unknown. Here we present the cryogenic-electron microscopy structures of the Deinococcus radiodurans TnpB-reRNA (right-end transposon element-derived RNA) complex in DNA-bound and -free forms. The structures reveal the basic architecture of TnpB nuclease and the molecular mechanism for DNA target recognition and cleavage that is supported by biochemical experiments. Collectively, these results demonstrate that TnpB represents the minimal structural and functional core of the Cas12 protein family and provide a framework for developing TnpB-based genome editing tools.

PubMed: 37020015
DOI: 10.1038/s41586-023-05826-x

実験手法

ELECTRON MICROSCOPY (3.14 Å)