8ETD

Crystal Structure of Schizosaccharomyces pombe Rho1

8ETD の概要

• エントリーDOI: 10.2210/pdb8etd/pdb
• 分子名称: GTP-binding protein rho1, MAGNESIUM ION, GUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: gtpase, rho, gdp, evolution, gene regulation
• 由来する生物種: Schizosaccharomyces pombe (strain 972 / ATCC 24843)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46045.04

構造登録者

Huang, Q.,Xie, J.,Seetharaman, J. (登録日: 2022-10-16, 公開日: 2022-12-07, 最終更新日: 2023-10-25)

主引用文献

Huang, Q.,Xie, J.,Seetharaman, J.
Crystal Structure of Schizosaccharomyces pombe Rho1 Reveals Its Evolutionary Relationship with Other Rho GTPases.
Biology (Basel), 11:-, 2022

PubMed Abstract: The Rho protein, a homolog of Ras, is a member of the Ras superfamily of small GTPases. Rho family proteins are involved in cytoskeletal organization, cell mobility, and polarity, and are implicated in cancer morphogenesis. Although Rho homologs from higher-order mammalian organisms are well studied, there are few studies examining Rho proteins in lower-level single-celled organisms. Here, we report on the crystal structure of Rho1 from (Rho1) in complex with GDP in the presence of Mg at a 2.78 Å resolution. The overall structure is similar to that of known Rho homologs, including human RhoA, human RhoC, and Rho1 (Rho1), with some exceptions. We observed subtle differences at the Switch I and II regions, in β2 and β3, and in the Rho insert domain and loop from Phe107 to Pro112. Our analysis suggests that Rho is evolutionarily closer to RhoC than RhoA, as previously believed.

PubMed: 36358328
DOI: 10.3390/biology11111627

実験手法

X-RAY DIFFRACTION (2.78 Å)