8ENV

In situ cryo-EM structure of Pseudomonas phage E217 tail baseplate in C6 map

8ENV の概要

• エントリーDOI: 10.2210/pdb8env/pdb
• EMDBエントリー: 28280
• 分子名称: Sheath protein gp31, Structural protein gp33, Sheath initiator gp34, ... (6 entities in total)
• 機能のキーワード: pseudomonas, phage, baseplate, virus
• 由来する生物種: Pseudomonas phage vB_PaeM_E217; 詳細
• タンパク質・核酸の鎖数: 36
• 化学式量合計: 1142790.43

構造登録者

Li, F.,Cingolani, G.,Hou, C. (登録日: 2022-09-30, 公開日: 2023-07-26, 最終更新日: 2024-06-19)

主引用文献

Li, F.,Hou, C.D.,Lokareddy, R.K.,Yang, R.,Forti, F.,Briani, F.,Cingolani, G.
High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217.
Nat Commun, 14:4052-4052, 2023

PubMed Abstract: E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after DNA ejection at 3.1 Å and 4.5 Å resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify and build de novo structures for 19 unique E217 gene products, resolve the tail genome-ejection machine in both extended and contracted states, and decipher the complete architecture of the baseplate formed by 66 polypeptide chains. We also determine that E217 recognizes the host O-antigen as a receptor, and we resolve the N-terminal portion of the O-antigen-binding tail fiber. We propose that E217 design principles presented in this paper are conserved across PB1-like Myoviridae phages of the Pbunavirus genus that encode a ~1.4 MDa baseplate, dramatically smaller than the coliphage T4.

PubMed: 37422479
DOI: 10.1038/s41467-023-39756-z

実験手法

ELECTRON MICROSCOPY (3.42 Å)