8EM2

Cryo-EM structure of the human GDH/6PGL endoplasmic bifunctional protein

8EM2 の概要

• エントリーDOI: 10.2210/pdb8em2/pdb
• EMDBエントリー: 23429 28232
• 分子名称: GDH/6PGL endoplasmic bifunctional protein, 2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total)
• 機能のキーワード: h6pd, oxidoreductase, hydrolase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 178445.57

構造登録者

Su, C.C. (登録日: 2022-09-26, 公開日: 2023-05-03, 最終更新日: 2024-11-13)

主引用文献

Su, C.C.,Lyu, M.,Zhang, Z.,Miyagi, M.,Huang, W.,Taylor, D.J.,Yu, E.W.
High-resolution structural-omics of human liver enzymes.
Cell Rep, 42:112609-112609, 2023

PubMed Abstract: We applied raw human liver microsome lysate to a holey carbon grid and used cryo-electron microscopy (cryo-EM) to define its composition. From this sample we identified and simultaneously determined high-resolution structural information for ten unique human liver enzymes involved in diverse cellular processes. Notably, we determined the structure of the endoplasmic bifunctional protein H6PD, where the N- and C-terminal domains independently possess glucose-6-phosphate dehydrogenase and 6-phosphogluconolactonase enzymatic activity, respectively. We also obtained the structure of heterodimeric human GANAB, an ER glycoprotein quality-control machinery that contains a catalytic α subunit and a noncatalytic β subunit. In addition, we observed a decameric peroxidase, PRDX4, which directly contacts a disulfide isomerase-related protein, ERp46. Structural data suggest that several glycosylations, bound endogenous compounds, and ions associate with these human liver enzymes. These results highlight the importance of cryo-EM in facilitating the elucidation of human organ proteomics at the atomic level.

PubMed: 37289586
DOI: 10.1016/j.celrep.2023.112609

実験手法

ELECTRON MICROSCOPY (3.02 Å)