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8EF2

Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ107-B

Summary for 8EF2
Entry DOI10.2210/pdb8ef2/pdb
DescriptorrhMZ107-B antibody heavy chain, rhMZ107-B antibody light chain, PHOSPHATE ION, ... (4 entities in total)
Functional Keywordszikv, zikv-specific, cross-protomer epitopes, viral protein, immune system
Biological sourceMacaca mulatta (Rhesus monkey)
More
Total number of polymer chains2
Total formula weight47893.03
Authors
Sankhala, R.S.,Joyce, M.G. (deposition date: 2022-09-07, release date: 2023-08-30, Last modification date: 2024-10-09)
Primary citationSankhala, R.S.,Dussupt, V.,Donofrio, G.,Gromowski, G.D.,De La Barrera, R.A.,Larocca, R.A.,Mendez-Rivera, L.,Lee, A.,Choe, M.,Zaky, W.,Mantus, G.,Jensen, J.L.,Chen, W.H.,Gohain, N.,Bai, H.,McCracken, M.K.,Mason, R.D.,Leggat, D.,Slike, B.M.,Tran, U.,Jian, N.,Abbink, P.,Peterson, R.,Mendes, E.A.,Freitas de Oliveira Franca, R.,Calvet, G.A.,Bispo de Filippis, A.M.,McDermott, A.,Roederer, M.,Hernandez, M.,Albertus, A.,Davidson, E.,Doranz, B.J.,Rolland, M.,Robb, M.L.,Lynch, R.M.,Barouch, D.H.,Jarman, R.G.,Thomas, S.J.,Modjarrad, K.,Michael, N.L.,Krebs, S.J.,Joyce, M.G.
Zika-specific neutralizing antibodies targeting inter-dimer envelope epitopes.
Cell Rep, 42:112942-112942, 2023
Cited by
PubMed Abstract: Zika virus (ZIKV) is an emerging pathogen that causes devastating congenital defects. The overlapping epidemiology and immunologic cross-reactivity between ZIKV and dengue virus (DENV) pose complex challenges to vaccine design, given the potential for antibody-dependent enhancement of disease. Therefore, classification of ZIKV-specific antibody targets is of notable value. From a ZIKV-infected rhesus macaque, we identify ZIKV-reactive B cells and isolate potent neutralizing monoclonal antibodies (mAbs) with no cross-reactivity to DENV. We group these mAbs into four distinct antigenic groups targeting ZIKV-specific cross-protomer epitopes on the envelope glycoprotein. Co-crystal structures of representative mAbs in complex with ZIKV envelope glycoprotein reveal envelope-dimer epitope and unique dimer-dimer epitope targeting. All four specificities are serologically identified in convalescent humans following ZIKV infection, and representative mAbs from all four groups protect against ZIKV replication in mice. These results provide key insights into ZIKV-specific antigenicity and have implications for ZIKV vaccine, diagnostic, and therapeutic development.
PubMed: 37561630
DOI: 10.1016/j.celrep.2023.112942
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.102 Å)
Structure validation

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건을2024-11-06부터공개중

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