8EC0

III2IV respiratory supercomplex from Saccharomyces cerevisiae cardiolipin-lacking mutant

8EC0 の概要

• エントリーDOI: 10.2210/pdb8ec0/pdb
• EMDBエントリー: 28011
• 分子名称: Cytochrome b-c1 complex subunit 1, mitochondrial, Cytochrome c1, heme protein, mitochondrial, Cytochrome c oxidase subunit 8, mitochondrial, ... (30 entities in total)
• 機能のキーワード: s.cerevisiae, respiratory supercomplex, phosphatidylglycerol, oxidoreductase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 30
• 化学式量合計: 735115.04

構造登録者

Hryc, C.F.,Mileykovskaya, E.,Baker, M.,Dowhan, W. (登録日: 2022-08-31, 公開日: 2023-05-24, 最終更新日: 2024-11-13)

主引用文献

Hryc, C.F.,Mallampalli, V.K.P.S.,Bovshik, E.I.,Azinas, S.,Fan, G.,Serysheva, I.I.,Sparagna, G.C.,Baker, M.L.,Mileykovskaya, E.,Dowhan, W.
Structural insights into cardiolipin replacement by phosphatidylglycerol in a cardiolipin-lacking yeast respiratory supercomplex.
Nat Commun, 14:2783-2783, 2023

PubMed Abstract: Cardiolipin is a hallmark phospholipid of mitochondrial membranes. Despite established significance of cardiolipin in supporting respiratory supercomplex organization, a mechanistic understanding of this lipid-protein interaction is still lacking. To address the essential role of cardiolipin in supercomplex organization, we report cryo-EM structures of a wild type supercomplex (IVIIIIV) and a supercomplex (IIIIV) isolated from a cardiolipin-lacking Saccharomyces cerevisiae mutant at 3.2-Å and 3.3-Å resolution, respectively, and demonstrate that phosphatidylglycerol in IIIIV occupies similar positions as cardiolipin in IVIIIIV. Lipid-protein interactions within these complexes differ, which conceivably underlies the reduced level of IVIIIIV and high levels of IIIIV and free III and IV in mutant mitochondria. Here we show that anionic phospholipids interact with positive amino acids and appear to nucleate a phospholipid domain at the interface between the individual complexes, which dampen charge repulsion and further stabilize interaction, respectively, between individual complexes.

PubMed: 37188665
DOI: 10.1038/s41467-023-38441-5

実験手法

ELECTRON MICROSCOPY (3.3 Å)