8EB5

Tandem of Hermes transposase BED domain in complex with the quasi palindrome of its transposon left-end

8EB5 の概要

• エントリーDOI: 10.2210/pdb8eb5/pdb
• 分子名称: Hermes transposase BED domain, Hermes transposon left-end subterminal repeats 1 and 2, ZINC ION, ... (5 entities in total)
• 機能のキーワード: bed domain, zinc-binding domain, transposase, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Musca domestica (house fly); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 19627.98

構造登録者

Lannes, L.,Dyda, F. (登録日: 2022-08-30, 公開日: 2023-08-02, 最終更新日: 2024-10-02)

主引用文献

Lannes, L.,Furman, C.M.,Hickman, A.B.,Dyda, F.
Zinc-finger BED domains drive the formation of the active Hermes transpososome by asymmetric DNA binding.
Nat Commun, 14:4470-4470, 2023

PubMed Abstract: The Hermes DNA transposon is a member of the eukaryotic hAT superfamily, and its transposase forms a ring-shaped tetramer of dimers. Our investigation, combining biochemical, crystallography and cryo-electron microscopy, and in-cell assays, shows that the full-length Hermes octamer extensively interacts with its transposon left-end through multiple BED domains of three Hermes protomers contributed by three dimers explaining the role of the unusual higher-order assembly. By contrast, the right-end is bound to no BED domains at all. Thus, this work supports a model in which Hermes multimerizes to gather enough BED domains to find its left-end among the abundant genomic DNA, facilitating the subsequent interaction with the right-end.

PubMed: 37491363
DOI: 10.1038/s41467-023-40210-3

実験手法

X-RAY DIFFRACTION (2.5 Å)