8E9A

Crystal structure of AsfvPolX in complex with 10-23 DNAzyme and Mg

8E9A の概要

• エントリーDOI: 10.2210/pdb8e9a/pdb
• 分子名称: Repair DNA polymerase X, DNA/RNA (52-MER), MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: dnazyme, dna, transferase-dna complex, transferase/dna
• 由来する生物種: African swine fever virus BA71V; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 73503.55

構造登録者

Cramer, E.R.,Robart, A.R.,Kaya, A.I. (登録日: 2022-08-26, 公開日: 2023-06-21, 最終更新日: 2024-10-09)

主引用文献

Cramer, E.R.,Starcovic, S.A.,Avey, R.M.,Kaya, A.I.,Robart, A.R.
Structure of a 10-23 deoxyribozyme exhibiting a homodimer conformation.
Commun Chem, 6:119-119, 2023

PubMed Abstract: Deoxyribozymes (DNAzymes) are in vitro evolved DNA sequences capable of catalyzing chemical reactions. The RNA-cleaving 10-23 DNAzyme was the first DNAzyme to be evolved and possesses clinical and biotechnical applications as a biosensor and a knockdown agent. DNAzymes do not require the recruitment of other components to cleave RNA and can turnover, thus they have a distinct advantage over other knockdown methods (siRNA, CRISPR, morpholinos). Despite this, a lack of structural and mechanistic information has hindered the optimization and application of the 10-23 DNAzyme. Here, we report a 2.7 Å crystal structure of the RNA-cleaving 10-23 DNAzyme in a homodimer conformation. Although proper coordination of the DNAzyme to substrate is observed along with intriguing patterns of bound magnesium ions, the dimer conformation likely does not capture the true catalytic form of the 10-23 DNAzyme.

PubMed: 37301907
DOI: 10.1038/s42004-023-00924-3

実験手法

X-RAY DIFFRACTION (2.69 Å)