8E4L
The open state mouse TRPM8 structure in complex with the cooling agonist C3, AITC, and PI(4,5)P2
Summary for 8E4L
| Entry DOI | 10.2210/pdb8e4l/pdb |
| EMDB information | 27891 |
| Descriptor | Transient receptor potential cation channel subfamily M member 8, CALCIUM ION, 3-isothiocyanatoprop-1-ene, ... (5 entities in total) |
| Functional Keywords | trpm8, menthol receptor, cold receptor, pi(4, 5)p2, cooling agonists, temperature sensing, ion channel, sensory transduction, transient receptor potential ion channel, membrane protein |
| Biological source | Mus musculus (house mouse) |
| Total number of polymer chains | 4 |
| Total formula weight | 530778.02 |
| Authors | Yin, Y.,Zhang, F.,Feng, S.,Butay, K.J.,Borgnia, M.J.,Im, W.,Lee, S.-Y. (deposition date: 2022-08-18, release date: 2022-10-26, Last modification date: 2024-10-16) |
| Primary citation | Yin, Y.,Zhang, F.,Feng, S.,Butay, K.J.,Borgnia, M.J.,Im, W.,Lee, S.Y. Activation mechanism of the mouse cold-sensing TRPM8 channel by cooling agonist and PIP 2. Science, 378:eadd1268-eadd1268, 2022 Cited by PubMed Abstract: The transient receptor potential melastatin 8 (TRPM8) channel is the primary molecular transducer responsible for the cool sensation elicited by menthol and cold in mammals. TRPM8 activation is controlled by cooling compounds together with the membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP). Our knowledge of cold sensation and the therapeutic potential of TRPM8 for neuroinflammatory diseases and pain will be enhanced by understanding the structural basis of cooling agonist- and PIP-dependent TRPM8 activation. We present cryo-electron microscopy structures of mouse TRPM8 in closed, intermediate, and open states along the ligand- and PIP-dependent gating pathway. Our results uncover two discrete agonist sites, state-dependent rearrangements in the gate positions, and a disordered-to-ordered transition of the gate-forming S6-elucidating the molecular basis of chemically induced cool sensation in mammals. PubMed: 36227998DOI: 10.1126/science.add1268 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.32 Å) |
Structure validation
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