8DXS

Cryo-EM structure of RBD-directed neutralizing antibody P2B4 in complex with prefusion SARS-CoV-2 spike glycoprotein

8DXS の概要

• エントリーDOI: 10.2210/pdb8dxs/pdb
• EMDBエントリー: 27775
• 分子名称: Spike glycoprotein, P2B4 Heavy chain, P2B4 Light chain, ... (4 entities in total)
• 機能のキーワード: neutralizing antibody, fusion protein, spike glycoprotein, covid-19, rbd, rbd-directed antibody, p2b4, viral protein, immune system, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 578863.38

構造登録者

Reddem, E.R.,Shapiro, L. (登録日: 2022-08-03, 公開日: 2022-12-07, 最終更新日: 2024-10-23)

主引用文献

Luo, M.,Zhou, B.,Reddem, E.R.,Tang, B.,Chen, B.,Zhou, R.,Liu, H.,Liu, L.,Katsamba, P.S.,Au, K.K.,Man, H.O.,To, K.K.,Yuen, K.Y.,Shapiro, L.,Dang, S.,Ho, D.D.,Chen, Z.
Structural insights into broadly neutralizing antibodies elicited by hybrid immunity against SARS-CoV-2.
Emerg Microbes Infect, 12:2146538-2146538, 2023

PubMed Abstract: Increasing spread by SARS-CoV-2 Omicron variants challenges existing vaccines and broadly reactive neutralizing antibodies (bNAbs) against COVID-19. Here we determine the diversity, potency, breadth and structural insights of bNAbs derived from memory B cells of BNT162b2-vaccinee after homogeneous Omicron BA.1 breakthrough infection. The infection activates diverse memory B cell clonotypes for generating potent class I/II and III bNAbs with new epitopes mapped to the receptor-binding domain (RBD). The top eight bNAbs neutralize wildtype and BA.1 potently but display divergent IgH/IgL sequences and neuralization profiles against other variants of concern (VOCs). Two of them (P2D9 and P3E6) belonging to class III NAbs display comparable potency against BA.4/BA.5, although structural analysis reveals distinct modes of action. P3E6 neutralizes all variants tested through a unique bivalent interaction with two RBDs. Our findings provide new insights into hybrid immunity on BNT162b2-induced diverse memory B cells in response to Omicron breakthrough infection for generating diverse bNAbs with distinct structural basis.

PubMed: 36354024
DOI: 10.1080/22221751.2022.2146538

実験手法

ELECTRON MICROSCOPY (3.76 Å)