8DX4

Clostridioides difficile R20291 minor pilin - PilW fused with Maltose Binding Protein

8DX4 の概要

• エントリーDOI: 10.2210/pdb8dx4/pdb
• 分子名称: Maltodextrin-binding protein,Putative pilin protein, 1,2-ETHANEDIOL, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ... (6 entities in total)
• 機能のキーワード: membrane bound protein, pilin, dna binding protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 225737.46

構造登録者

Ronish, L.A.,Piepenbrink, K. (登録日: 2022-08-02, 公開日: 2022-09-21, 最終更新日: 2023-10-18)

主引用文献

Ronish, L.A.,Sidner, B.,Yu, Y.,Piepenbrink, K.H.
Recognition of extracellular DNA by type IV pili promotes biofilm formation by Clostridioides difficile.
J.Biol.Chem., 298:102449-102449, 2022

PubMed Abstract: Clostridioides difficile is a Gram-positive bacillus, which is a frequent cause of gastrointestinal infections triggered by the depletion of the gut microbiome. Because of the frequent recurrence of these infections after antibiotic treatment, mechanisms of C. difficile persistence and recurrence, including biofilm formation, are of increasing interest. Previously, our group and others found that type IV pili, filamentous helical appendages polymerized from protein subunits, promoted microcolony and biofilm formation in C. difficile. In Gram-negative bacteria, the ability of type IV pili to mediate bacterial self-association has been explained through interactions between the pili of adjacent cells, but type IV pili from several Gram-negative species are also required for natural competence through DNA uptake. Here, we report the ability of two C. difficile pilin subunits, PilJ and PilW, to bind to DNA in vitro, as well as the defects in biofilm formation in the pilJ and pilW gene-interruption mutants. Additionally, we have resolved the X-ray crystal structure of PilW, which we use to model possible structural mechanisms for the formation of C. difficile biofilm through interactions between type IV pili and the DNA of the extracellular matrix. Taken together, our results provide further insight into the relationship between type IV pilus function and biofilm formation in C. difficile and, more broadly, suggest that DNA recognition by type IV pili and related structures may have functional importance beyond DNA uptake for natural competence.

PubMed: 36064001
DOI: 10.1016/j.jbc.2022.102449

実験手法

X-RAY DIFFRACTION (2.487 Å)