8DE5

Structure of glyceraldehyde-3-phosphate dehydrogenase from Paracoccidioides lutzii

8DE5 の概要

• エントリーDOI: 10.2210/pdb8de5/pdb
• 分子名称: Glyceraldehyde-3-phosphate dehydrogenase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, D-galactonic acid, ... (6 entities in total)
• 機能のキーワード: glyceraldehyde-3-phosphate dehydrogenase, paracoccidioides lutzii, paracoccidioidomycosis, aldonic sugar acid, oxidoreductase
• 由来する生物種: Paracoccidioides lutzii Pb01
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38056.57

構造登録者

Hernandez-Prieto, J.H.,Martini, V.P.,Iulek, J. (登録日: 2022-06-19, 公開日: 2023-06-21, 最終更新日: 2023-12-06)

主引用文献

Hernandez-Prieto, J.H.,Martini, V.P.,Iulek, J.
Structure of glyceraldehyde-3-phosphate dehydrogenase from Paracoccidioides lutzii in complex with an aldonic sugar acid.
Biochimie, 218:20-33, 2023

PubMed Abstract: The pathogen Paracoccidioides lutzii (Pb01) is found in South America countries Colombia, Ecuador, Venezuela and Brazil, especially in the central, west, and north regions of the latter. It belongs to the Ajellomycetaceae family, Onygenales order, and is typically thermodimorphic, presenting yeast cells when it grows in animal tissues, but mycelia when in the environment, where it produces the infectious propagule. This fungus is one of the etiologic agents of Paracoccidioidomycosis (PCM), the most important endemic fungal infection in Latin America. Investigations on its genome have contributed to a better understanding about its metabolism and revealed the complexity of several metabolic glycolytic pathways. Glyceraldehyde-3-Phosphate Dehydrogenase from Paracoccidioides lutzii (PlGAPDH) is considered a moonlighting protein and participates in several biological processes of this pathogen. The enzyme was expressed and purified, as seen in SDS-PAGE gel, crystallized and had its three dimensional structure (3D) determined in complex with NAD, a sulphate ion and d-galactonic acid, therefore, a type of 'GAA site'. It is the first GAPDH structure to show this chemical type in this site and how this protein can bind an acid derived from oxidation of a linear hexose.

PubMed: 37709188
DOI: 10.1016/j.biochi.2023.09.013

実験手法

X-RAY DIFFRACTION (2.02 Å)