8DDC

Intramembrane recognition between transmembrane domains of IL-7R and common gamma chain

8DDC の概要

• エントリーDOI: 10.2210/pdb8ddc/pdb
• NMR情報: BMRB: 31026
• 分子名称: Cytokine receptor common subunit gamma, Interleukin-7 receptor subunit alpha (2 entities in total)
• 機能のキーワード: common gamma-chain cytokine receptor, transmembrane domain, receptor sharing, membrane protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7316.75

構造登録者

Cai, T.,Lenoir Capello, R.,Chou, J.J. (登録日: 2022-06-17, 公開日: 2023-07-05, 最終更新日: 2024-05-15)

主引用文献

Cai, T.,Lenoir Capello, R.,Pi, X.,Wu, H.,Chou, J.J.
Structural basis of gamma chain family receptor sharing at the membrane level.
Science, 381:569-576, 2023

PubMed Abstract: Common γ chain (γc) cytokine receptors, including interleukin-2 (IL-2), IL-4, IL-7, IL-9, IL-15, and IL-21 receptors, are activated upon engagement with a common γc receptor (CD132) by concomitant binding of their ectodomains to an interleukin. In this work, we find that direct interactions between the transmembrane domains (TMDs) of both the γc and the interleukin receptors (ILRs) are also required for receptor activation. Moreover, the same γc TMD can specifically recognize multiple ILR TMDs of diverse sequences within the family. Heterodimer structures of γc TMD bound to IL-7 and IL-9 receptor TMDs-determined in a lipid bilayer-like environment by nuclear magnetic resonance spectroscopy-reveal a conserved knob-into-hole mechanism of recognition that mediates receptor sharing within the membrane. Thus, signaling in the γc receptor family requires specific heterotypic interactions of the TMDs.

PubMed: 37535730
DOI: 10.1126/science.add1219

実験手法

SOLUTION NMR