8DC2

Cryo-EM structure of CasLambda (Cas12l) bound to crRNA and DNA

8DC2 の概要

• エントリーDOI: 10.2210/pdb8dc2/pdb
• EMDBエントリー: 27320
• 分子名称: CasLambda, RNA (51-MER), DNA TS, ... (4 entities in total)
• 機能のキーワード: crispr, rna binding protein, dna binding protein, phage, viral protein, enzyme, ribonucleoprotein, rna binding protein-rna-dna complex, rna binding protein/rna/dna
• 由来する生物種: uncultured virus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 132489.14

構造登録者

Al-Shayeb, B.,Skopintsev, P.,Soczek, K.,Doudna, J. (登録日: 2022-06-15, 公開日: 2022-12-14, 最終更新日: 2024-06-12)

主引用文献

Al-Shayeb, B.,Skopintsev, P.,Soczek, K.M.,Stahl, E.C.,Li, Z.,Groover, E.,Smock, D.,Eggers, A.R.,Pausch, P.,Cress, B.F.,Huang, C.J.,Staskawicz, B.,Savage, D.F.,Jacobsen, S.E.,Banfield, J.F.,Doudna, J.A.
Diverse virus-encoded CRISPR-Cas systems include streamlined genome editors.
Cell, 185:4574-4586.e16, 2022

PubMed Abstract: CRISPR-Cas systems are host-encoded pathways that protect microbes from viral infection using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find that CRISPR systems are also encoded in diverse bacteriophages, where they occur as divergent and hypercompact anti-viral systems. Bacteriophage-encoded CRISPR systems belong to all six known CRISPR-Cas types, though some lack crucial components, suggesting alternate functional roles or host complementation. We describe multiple new Cas9-like proteins and 44 families related to type V CRISPR-Cas systems, including the Casλ RNA-guided nuclease family. Among the most divergent of the new enzymes identified, Casλ recognizes double-stranded DNA using a uniquely structured CRISPR RNA (crRNA). The Casλ-RNA-DNA structure determined by cryoelectron microscopy reveals a compact bilobed architecture capable of inducing genome editing in mammalian, Arabidopsis, and hexaploid wheat cells. These findings reveal a new source of CRISPR-Cas enzymes in phages and highlight their value as genome editors in plant and human cells.

PubMed: 36423580
DOI: 10.1016/j.cell.2022.10.020

実験手法

ELECTRON MICROSCOPY (2.99 Å)