8CYM

[2T7+9bp Linker] Self-Assembled 3D DNA Hexagonal Tensegrity Triangle with 9 bp Sticky-End Linker

8CYM の概要

• エントリーDOI: 10.2210/pdb8cym/pdb
• 関連するPDBエントリー: 7R96
• 分子名称: DNA (5'-D(*AP*CP*GP*CP*AP*GP*CP*CP*TP*GP*TP*AP*CP*GP*GP*AP*CP*AP*TP*C)-3'), DNA (5'-D(P*CP*CP*GP*TP*AP*CP*A)-3'), DNA (5'-D(P*GP*GP*CP*TP*GP*C)-3'), ... (6 entities in total)
• 機能のキーワード: tensegrity triangle, synthetic construct, self-assembly, dna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 18269.04

構造登録者

Lu, B.,Vecchioni, S.,Ohayon, Y.P.,Seeman, N.C.,Mao, C.,Sha, R. (登録日: 2022-05-24, 公開日: 2023-01-18, 最終更新日: 2023-10-25)

主引用文献

Lu, B.,Woloszyn, K.,Ohayon, Y.P.,Yang, B.,Zhang, C.,Mao, C.,Seeman, N.C.,Vecchioni, S.,Sha, R.
Programmable 3D Hexagonal Geometry of DNA Tensegrity Triangles.
Angew.Chem.Int.Ed.Engl., 62:e202213451-e202213451, 2023

PubMed Abstract: Non-canonical interactions in DNA remain under-explored in DNA nanotechnology. Recently, many structures with non-canonical motifs have been discovered, notably a hexagonal arrangement of typically rhombohedral DNA tensegrity triangles that forms through non-canonical sticky end interactions. Here, we find a series of mechanisms to program a hexagonal arrangement using: the sticky end sequence; triangle edge torsional stress; and crystallization condition. We showcase cross-talking between Watson-Crick and non-canonical sticky ends in which the ratio between the two dictates segregation by crystal forms or combination into composite crystals. Finally, we develop a method for reconfiguring the long-range geometry of formed crystals from rhombohedral to hexagonal and vice versa. These data demonstrate fine control over non-canonical motifs and their topological self-assembly. This will vastly increase the programmability, functionality, and versatility of rationally designed DNA constructs.

PubMed: 36520622
DOI: 10.1002/anie.202213451

実験手法

X-RAY DIFFRACTION (7.76 Å)