8CXJ

The IgI3 domain of R28 protein from S. pyogenes bound to CEACAM1

Summary for 8CXJ

• Entry DOI: 10.2210/pdb8cxj/pdb
• Descriptor: Carcinoembryonic antigen-related cell adhesion molecule 1, Surface protein R28, GLYCEROL, ... (6 entities in total)
• Functional Keywords: bacterial, adhesion, cell adhesion
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 8
• Total formula weight: 105483.85

Authors

Bonsor, D.A.,McCarthy, A.J. (deposition date: 2022-05-21, release date: 2023-03-15, Last modification date: 2023-10-25)

Primary citation

Catton, E.A.,Bonsor, D.A.,Herrera, C.,Stalhammar-Carlemalm, M.,Lyndin, M.,Turner, C.E.,Soden, J.,van Strijp, J.A.G.,Singer, B.B.,van Sorge, N.M.,Lindahl, G.,McCarthy, A.J.
Human CEACAM1 is targeted by a Streptococcus pyogenes adhesin implicated in puerperal sepsis pathogenesis.
Nat Commun, 14:2275-2275, 2023

PubMed Abstract: Life-threatening bacterial infections in women after childbirth, known as puerperal sepsis, resulted in classical epidemics and remain a global health problem. While outbreaks of puerperal sepsis have been ascribed to Streptococcus pyogenes, little is known about disease mechanisms. Here, we show that the bacterial R28 protein, which is epidemiologically associated with outbreaks of puerperal sepsis, specifically targets the human receptor CEACAM1. This interaction triggers events that would favor the development of puerperal sepsis, including adhesion to cervical cells, suppression of epithelial wound repair and subversion of innate immune responses. High-resolution structural analysis showed that an R28 domain with IgI3-like fold binds to the N-terminal domain of CEACAM1. Together, these findings demonstrate that a single adhesin-receptor interaction can drive the pathogenesis of bacterial sepsis and provide molecular insights into the pathogenesis of one of the most important infectious diseases in medical history.

PubMed: 37080973
DOI: 10.1038/s41467-023-37732-1

Experimental method

X-RAY DIFFRACTION (3.05 Å)