8COG

Human arginylated beta-actin

8COG の概要

• エントリーDOI: 10.2210/pdb8cog/pdb
• EMDBエントリー: 16776
• 分子名称: Actin, cytoplasmic 1, N-terminally processed, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: actin, methylated, filament, contractile protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42158.10

構造登録者

Pinto, C.S.,Bakker, S.E.,Suchenko, A.,Hussain, H.,Hatano, T.,Sampath, K.,Chinthalapudi, K.,Mishima, M.,Balasubramanian, M. (登録日: 2023-02-28, 公開日: 2024-03-06, 最終更新日: 2025-12-17)

主引用文献

Pinto, C.S.,Bakker, S.E.,Suchenko, A.,Kolodny, I.M.,Hussain, H.,Hatano, T.,Sampath, K.,Chinthalapudi, K.,Heissler, S.M.,Mishima, M.,Balasubramanian, M.
Actin arginylation alters myosin engagement and F-actin patterning despite structural conservation.
J.Cell Biol., 225:-, 2026

PubMed Abstract: Actin is a conserved protein with crucial roles in cell polarity, division, and muscle contraction. Its function is regulated in part by posttranslational modifications, one of which is N-terminal arginylation. What is the structure of arginylated-β-actin (R-β-actin), and how does it regulate F-actin function? Here we report the 3.6 Å structures of ADP-R-β-actin filaments, which are nearly identical to that of non-arginylated F-actin. In vitro assays reveal that the interaction between myosin-II and actin is altered upon actin arginylation, characterized by frequent detachment of R-actin filaments from myosin-II. In vivo, replacement of the only actin gene in Schizosaccharomyces pombe with a synthetic gene encoding R-Sp-actin reduces Arp2/3-based actin patches while thickening formin-induced actin cables. Consistent with defective interactions between myosin-II and R-actin filaments, assembly and constriction of the cytokinetic actomyosin ring are perturbed in R-Sp-actin cells. Thus, despite structural similarity of arginylated and non-arginylated actin filaments, actin arginylation affects F-actin assortment into distinct subcellular structures and its interaction with myosin-II.

PubMed: 41236477
DOI: 10.1083/jcb.202409067

実験手法

ELECTRON MICROSCOPY (3.499 Å)