8CLL

Structural insights into human TFIIIC promoter recognition

Summary for 8CLL

• Entry DOI: 10.2210/pdb8cll/pdb
• EMDB information: 16717
• Descriptor: General transcription factor 3C polypeptide 1, General transcription factor 3C polypeptide 4, General transcription factor 3C polypeptide 2, ... (4 entities in total)
• Functional Keywords: tfiiic, trna gene, b-box promoter, dna recognition, transcription
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 6
• Total formula weight: 878074.37

Authors

Seifert-Davila, W.,Girbig, M.,Hauptmann, L.,Hoffmann, T.,Eustermann, S.,Mueller, C.W. (deposition date: 2023-02-16, release date: 2023-06-21, Last modification date: 2023-12-20)

Primary citation

Seifert-Davila, W.,Girbig, M.,Hauptmann, L.,Hoffmann, T.,Eustermann, S.,Muller, C.W.
Structural insights into human TFIIIC promoter recognition.
Sci Adv, 9:eadh2019-eadh2019, 2023

PubMed Abstract: Transcription factor (TF) IIIC recruits RNA polymerase (Pol) III to most of its target genes. Recognition of intragenic A- and B-box motifs in transfer RNA (tRNA) genes by TFIIIC modules τA and τB is the first critical step for tRNA synthesis but is mechanistically poorly understood. Here, we report cryo-electron microscopy structures of the six-subunit human TFIIIC complex unbound and bound to a tRNA gene. The τB module recognizes the B-box via DNA shape and sequence readout through the assembly of multiple winged-helix domains. TFIIIC220 forms an integral part of both τA and τB connecting the two subcomplexes via a ~550-amino acid residue flexible linker. Our data provide a structural mechanism by which high-affinity B-box recognition anchors TFIIIC to promoter DNA and permits scanning for low-affinity A-boxes and TFIIIB for Pol III activation.

PubMed: 37418517
DOI: 10.1126/sciadv.adh2019

Experimental method

ELECTRON MICROSCOPY (3.4 Å)