8CLL

Structural insights into human TFIIIC promoter recognition

8CLL の概要

• エントリーDOI: 10.2210/pdb8cll/pdb
• EMDBエントリー: 16717
• 分子名称: General transcription factor 3C polypeptide 1, General transcription factor 3C polypeptide 4, General transcription factor 3C polypeptide 2, ... (4 entities in total)
• 機能のキーワード: tfiiic, trna gene, b-box promoter, dna recognition, transcription
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 878074.37

構造登録者

Seifert-Davila, W.,Girbig, M.,Hauptmann, L.,Hoffmann, T.,Eustermann, S.,Mueller, C.W. (登録日: 2023-02-16, 公開日: 2023-06-21, 最終更新日: 2023-12-20)

主引用文献

Seifert-Davila, W.,Girbig, M.,Hauptmann, L.,Hoffmann, T.,Eustermann, S.,Muller, C.W.
Structural insights into human TFIIIC promoter recognition.
Sci Adv, 9:eadh2019-eadh2019, 2023

PubMed Abstract: Transcription factor (TF) IIIC recruits RNA polymerase (Pol) III to most of its target genes. Recognition of intragenic A- and B-box motifs in transfer RNA (tRNA) genes by TFIIIC modules τA and τB is the first critical step for tRNA synthesis but is mechanistically poorly understood. Here, we report cryo-electron microscopy structures of the six-subunit human TFIIIC complex unbound and bound to a tRNA gene. The τB module recognizes the B-box via DNA shape and sequence readout through the assembly of multiple winged-helix domains. TFIIIC220 forms an integral part of both τA and τB connecting the two subcomplexes via a ~550-amino acid residue flexible linker. Our data provide a structural mechanism by which high-affinity B-box recognition anchors TFIIIC to promoter DNA and permits scanning for low-affinity A-boxes and TFIIIB for Pol III activation.

PubMed: 37418517
DOI: 10.1126/sciadv.adh2019

実験手法

ELECTRON MICROSCOPY (3.4 Å)