8CEO

Yeast RNA polymerase II transcription pre-initiation complex with core Mediator and the +1 nucleosome

これはPDB形式変換不可エントリーです。

8CEO の概要

• エントリーDOI: 10.2210/pdb8ceo/pdb
• EMDBエントリー: 16611
• 分子名称: General transcription and DNA repair factor IIH helicase subunit XPD, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II subunit RPB3, ... (53 entities in total)
• 機能のキーワード: pic, mediator, nucleosome, tf, transcription
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 54
• 化学式量合計: 2102672.10

構造登録者

Wang, H.,Cramer, P. (登録日: 2023-02-02, 公開日: 2023-03-08, 最終更新日: 2024-07-24)

主引用文献

Schilbach, S.,Wang, H.,Dienemann, C.,Cramer, P.
Yeast PIC-Mediator structure with RNA polymerase II C-terminal domain.
Proc.Natl.Acad.Sci.USA, 120:e2220542120-e2220542120, 2023

PubMed Abstract: For transcription initiation, RNA polymerase II (Pol II) forms a preinitiation complex (PIC) that associates with the general coactivator Mediator. Whereas atomic models of the human PIC-Mediator structure have been reported, structures for its yeast counterpart remain incomplete. Here, we present an atomic model for the yeast PIC with core Mediator, including the Mediator middle module that was previously poorly resolved and including subunit Med1 that was previously lacking. We observe three peptide regions containing eleven of the 26 heptapeptide repeats of the flexible C-terminal repeat domain (CTD) of Pol II. Two of these CTD regions bind between the Mediator head and middle modules and form defined CTD-Mediator interactions. CTD peptide 1 binds between the Med6 shoulder and Med31 knob domains, whereas CTD peptide 2 forms additional contacts with Med4. The third CTD region (peptide 3) binds in the Mediator cradle and associates with the Mediator hook. Comparisons with the human PIC-Mediator structure show that the central region in peptide 1 is similar and forms conserved contacts with Mediator, whereas peptides 2 and 3 exhibit distinct structures and Mediator interactions.

PubMed: 37014863
DOI: 10.1073/pnas.2220542120

実験手法

ELECTRON MICROSCOPY (3.6 Å)