8CBO

Structure of human mitochondrial MRPP1-MRPP2 in complex with mitochondrial pre-tRNA-Ile

8CBO の概要

• エントリーDOI: 10.2210/pdb8cbo/pdb
• 関連するPDBエントリー: 8CBK 8CBL 8CBM
• EMDBエントリー: 16547 16692
• 分子名称: 3-hydroxyacyl-CoA dehydrogenase type-2, tRNA methyltransferase 10 homolog C, Mitochondrial Precursor tRNA-Ile(5,4), ... (5 entities in total)
• 機能のキーワード: rna maturation, rna modification, mitochondrial trna, rna methyltransferase, mrpp1, mrpp2, mrpp3, rna binding protein, m6a, rna binding, rna recognition
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 158383.89

構造登録者

MEYNIER, V.,HARDWICK, S.,CATALA, M.,ROSKE, J.,OERUM, S.,CHIRGADZE, D.,BARRAUD, P.,LUISI, B.,TISNE, C. (登録日: 2023-01-25, 公開日: 2024-06-12, 最終更新日: 2025-12-10)

主引用文献

Meynier, V.,Hardwick, S.W.,Catala, M.,Roske, J.J.,Oerum, S.,Chirgadze, D.Y.,Barraud, P.,Yue, W.W.,Luisi, B.F.,Tisne, C.
Structural basis for human mitochondrial tRNA maturation.
Nat Commun, 15:4683-4683, 2024

PubMed Abstract: The human mitochondrial genome is transcribed into two RNAs, containing mRNAs, rRNAs and tRNAs, all dedicated to produce essential proteins of the respiratory chain. The precise excision of tRNAs by the mitochondrial endoribonucleases (mt-RNase), P and Z, releases all RNA species from the two RNA transcripts. The tRNAs then undergo 3'-CCA addition. In metazoan mitochondria, RNase P is a multi-enzyme assembly that comprises the endoribonuclease PRORP and a tRNA methyltransferase subcomplex. The requirement for this tRNA methyltransferase subcomplex for mt-RNase P cleavage activity, as well as the mechanisms of pre-tRNA 3'-cleavage and 3'-CCA addition, are still poorly understood. Here, we report cryo-EM structures that visualise four steps of mitochondrial tRNA maturation: 5' and 3' tRNA-end processing, methylation and 3'-CCA addition, and explain the defined sequential order of the tRNA processing steps. The methyltransferase subcomplex recognises the pre-tRNA in a distinct mode that can support tRNA-end processing and 3'-CCA addition, likely resulting from an evolutionary adaptation of mitochondrial tRNA maturation complexes to the structurally-fragile mitochondrial tRNAs. This subcomplex can also ensure a tRNA-folding quality-control checkpoint before the sequential docking of the maturation enzymes. Altogether, our study provides detailed molecular insight into RNA-transcript processing and tRNA maturation in human mitochondria.

PubMed: 38824131
DOI: 10.1038/s41467-024-49132-0

実験手法

ELECTRON MICROSCOPY (3.2 Å)