8C8Q

Cytochrome c oxidase from Schizosaccharomyces pombe

Summary for 8C8Q

• Entry DOI: 10.2210/pdb8c8q/pdb
• EMDB information: 16491
• Descriptor: Cytochrome c oxidase subunit 1, Cytochrome c oxidase subunit 12, mitochondrial, Cytochrome c oxidase subunit 13, mitochondrial, ... (20 entities in total)
• Functional Keywords: electron transfer, proton transfer, membrane protein, respiration
• Biological source: Schizosaccharomyces pombe (fission yeast); More
• Total number of polymer chains: 13
• Total formula weight: 257211.38

Authors

Moe, A.,Adelroth, P.,Brzezinski, P.,Nasvik Ojemyr, L. (deposition date: 2023-01-20, release date: 2023-03-01, Last modification date: 2024-11-20)

Primary citation

Moe, A.,Adelroth, P.,Brzezinski, P.,Nasvik Ojemyr, L.
Cryo-EM structure and function of S. pombe complex IV with bound respiratory supercomplex factor.
Commun Chem, 6:32-32, 2023

PubMed Abstract: Fission yeast Schizosaccharomyces pombe serves as model organism for studying higher eukaryotes. We combined the use of cryo-EM and spectroscopy to investigate the structure and function of affinity purified respiratory complex IV (CIV) from S. pombe. The reaction sequence of the reduced enzyme with O proceeds over a time scale of µs-ms, similar to that of the mammalian CIV. The cryo-EM structure of CIV revealed eleven subunits as well as a bound hypoxia-induced gene 1 (Hig1) domain of respiratory supercomplex factor 2 (Rcf2). These results suggest that binding of Rcf2 does not require the presence of a CIII-CIV supercomplex, i.e. Rcf2 is a component of CIV. An AlphaFold-Multimer model suggests that the Hig1 domains of both Rcf1 and Rcf2 bind at the same site of CIV suggesting that their binding is mutually exclusive. Furthermore, the differential functional effect of Rcf1 or Rcf2 is presumably caused by interactions of CIV with their different non-Hig1 domain parts.

PubMed: 36797353
DOI: 10.1038/s42004-023-00827-3

Experimental method

ELECTRON MICROSCOPY (3.36 Å)