8C8Q

Cytochrome c oxidase from Schizosaccharomyces pombe

8C8Q の概要

• エントリーDOI: 10.2210/pdb8c8q/pdb
• EMDBエントリー: 16491
• 分子名称: Cytochrome c oxidase subunit 1, Cytochrome c oxidase subunit 12, mitochondrial, Cytochrome c oxidase subunit 13, mitochondrial, ... (20 entities in total)
• 機能のキーワード: electron transfer, proton transfer, membrane protein, respiration
• 由来する生物種: Schizosaccharomyces pombe (fission yeast); 詳細
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 257211.38

構造登録者

Moe, A.,Adelroth, P.,Brzezinski, P.,Nasvik Ojemyr, L. (登録日: 2023-01-20, 公開日: 2023-03-01, 最終更新日: 2024-11-20)

主引用文献

Moe, A.,Adelroth, P.,Brzezinski, P.,Nasvik Ojemyr, L.
Cryo-EM structure and function of S. pombe complex IV with bound respiratory supercomplex factor.
Commun Chem, 6:32-32, 2023

PubMed Abstract: Fission yeast Schizosaccharomyces pombe serves as model organism for studying higher eukaryotes. We combined the use of cryo-EM and spectroscopy to investigate the structure and function of affinity purified respiratory complex IV (CIV) from S. pombe. The reaction sequence of the reduced enzyme with O proceeds over a time scale of µs-ms, similar to that of the mammalian CIV. The cryo-EM structure of CIV revealed eleven subunits as well as a bound hypoxia-induced gene 1 (Hig1) domain of respiratory supercomplex factor 2 (Rcf2). These results suggest that binding of Rcf2 does not require the presence of a CIII-CIV supercomplex, i.e. Rcf2 is a component of CIV. An AlphaFold-Multimer model suggests that the Hig1 domains of both Rcf1 and Rcf2 bind at the same site of CIV suggesting that their binding is mutually exclusive. Furthermore, the differential functional effect of Rcf1 or Rcf2 is presumably caused by interactions of CIV with their different non-Hig1 domain parts.

PubMed: 36797353
DOI: 10.1038/s42004-023-00827-3

実験手法

ELECTRON MICROSCOPY (3.36 Å)