8C80

Cryo-EM structure of the yeast SPT-Orm1-Monomer complex

8C80 の概要

• エントリーDOI: 10.2210/pdb8c80/pdb
• EMDBエントリー: 16467
• 分子名称: Protein ORM1, Serine palmitoyltransferase 1, Serine palmitoyltransferase 2, ... (8 entities in total)
• 機能のキーワード: serine-palmitoyl-transferase, spt, orm-protein, transferase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 166285.72

構造登録者

Schaefer, J.,Koerner, C.,Parey, K.,Januliene, D.,Moeller, A.,Froehlich, F. (登録日: 2023-01-18, 公開日: 2023-10-11)

主引用文献

Schafer, J.H.,Korner, C.,Esch, B.M.,Limar, S.,Parey, K.,Walter, S.,Januliene, D.,Moeller, A.,Frohlich, F.
Structure of the ceramide-bound SPOTS complex.
Nat Commun, 14:6196-6196, 2023

PubMed Abstract: Sphingolipids are structural membrane components that also function in cellular stress responses. The serine palmitoyltransferase (SPT) catalyzes the rate-limiting step in sphingolipid biogenesis. Its activity is tightly regulated through multiple binding partners, including Tsc3, Orm proteins, ceramides, and the phosphatidylinositol-4-phosphate (PI4P) phosphatase Sac1. The structural organization and regulatory mechanisms of this complex are not yet understood. Here, we report the high-resolution cryo-EM structures of the yeast SPT in complex with Tsc3 and Orm1 (SPOT) as dimers and monomers and a monomeric complex further carrying Sac1 (SPOTS). In all complexes, the tight interaction of the downstream metabolite ceramide and Orm1 reveals the ceramide-dependent inhibition. Additionally, observation of ceramide and ergosterol binding suggests a co-regulation of sphingolipid biogenesis and sterol metabolism within the SPOTS complex.

PubMed: 37794019
DOI: 10.1038/s41467-023-41747-z

実験手法

ELECTRON MICROSCOPY (3.4 Å)