8C4V

Hantaan virus polymerase in replication elongation state

8C4V の概要

• エントリーDOI: 10.2210/pdb8c4v/pdb
• 関連するPDBエントリー: 8C4S 8C4T 8C4U
• EMDBエントリー: 16427 16428 16429 16430
• 分子名称: RNA-directed RNA polymerase L, RNA (5'-R(P*AP*GP*GP*AP*GP*UP*AP*UP*CP*CP*AP*CP*CP*GP*CP*AP*AP*GP*A)-3'), RNA (5'-R(P*AP*CP*UP*AP*CP*UP*A)-3'), ... (5 entities in total)
• 機能のキーワード: polymerase, replication, bunyavirus, viral protein
• 由来する生物種: Hantaan virus 76-118; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 287534.45

構造登録者

Durieux trouilleton, Q.,Arragain, B.,Malet, H. (登録日: 2023-01-04, 公開日: 2023-06-07, 最終更新日: 2024-07-24)

主引用文献

Durieux Trouilleton, Q.,Barata-Garcia, S.,Arragain, B.,Reguera, J.,Malet, H.
Structures of active Hantaan virus polymerase uncover the mechanisms of Hantaviridae genome replication.
Nat Commun, 14:2954-2954, 2023

PubMed Abstract: Hantaviruses are causing life-threatening zoonotic infections in humans. Their tripartite negative-stranded RNA genome is replicated by the multi-functional viral RNA-dependent RNA-polymerase. Here we describe the structure of the Hantaan virus polymerase core and establish conditions for in vitro replication activity. The apo structure adopts an inactive conformation that involves substantial folding rearrangement of polymerase motifs. Binding of the 5' viral RNA promoter triggers Hantaan virus polymerase reorganization and activation. It induces the recruitment of the 3' viral RNA towards the polymerase active site for prime-and-realign initiation. The elongation structure reveals the formation of a template/product duplex in the active site cavity concomitant with polymerase core widening and the opening of a 3' viral RNA secondary binding site. Altogether, these elements reveal the molecular specificities of Hantaviridae polymerase structure and uncover the mechanisms underlying replication. They provide a solid framework for future development of antivirals against this group of emerging pathogens.

PubMed: 37221161
DOI: 10.1038/s41467-023-38555-w

実験手法

ELECTRON MICROSCOPY (3.14 Å)