8BZ1

RNA polymerase II core pre-initiation complex with the proximal +1 nucleosome (cPIC-Nuc10W)

これはPDB形式変換不可エントリーです。

8BZ1 の概要

• エントリーDOI: 10.2210/pdb8bz1/pdb
• 関連するPDBエントリー: 8BVW 8BYQ
• EMDBエントリー: 16274 16331 16335 16336 16337 16338
• 分子名称: DNA-directed RNA polymerase subunit, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11-a, ... (26 entities in total)
• 機能のキーワード: mammalian pic, +1 nucleosome, transcription initiation, transcription
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 970850.93

構造登録者

Abril-Garrido, J.,Dienemann, C.,Grabbe, F.,Velychko, T.,Lidschreiber, M.,Wang, H.,Cramer, P. (登録日: 2022-12-14, 公開日: 2023-05-03, 最終更新日: 2024-07-24)

主引用文献

Abril-Garrido, J.,Dienemann, C.,Grabbe, F.,Velychko, T.,Lidschreiber, M.,Wang, H.,Cramer, P.
Structural basis of transcription reduction by a promoter-proximal +1 nucleosome.
Mol.Cell, 83:1798-, 2023

PubMed Abstract: At active human genes, the +1 nucleosome is located downstream of the RNA polymerase II (RNA Pol II) pre-initiation complex (PIC). However, at inactive genes, the +1 nucleosome is found further upstream, at a promoter-proximal location. Here, we establish a model system to show that a promoter-proximal +1 nucleosome can reduce RNA synthesis in vivo and in vitro, and we analyze its structural basis. We find that the PIC assembles normally when the edge of the +1 nucleosome is located 18 base pairs (bp) downstream of the transcription start site (TSS). However, when the nucleosome edge is located further upstream, only 10 bp downstream of the TSS, the PIC adopts an inhibited state. The transcription factor IIH (TFIIH) shows a closed conformation and its subunit XPB contacts DNA with only one of its two ATPase lobes, inconsistent with DNA opening. These results provide a mechanism for nucleosome-dependent regulation of transcription initiation.

PubMed: 37148879
DOI: 10.1016/j.molcel.2023.04.011

実験手法

ELECTRON MICROSCOPY (3.8 Å)