8BV0

Binary complex between the NB-ARC domain from the Tomato immune receptor NRC1 and the SPRY domain-containing effector SS15 from the potato cyst nematode

8BV0 の概要

• エントリーDOI: 10.2210/pdb8bv0/pdb
• 分子名称: NRC1, Truncated secreted SPRY domain-containing protein 15 (Fragment), ADENOSINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: nlr, inhibitor, effector, sprysec, nematode, protein binding
• 由来する生物種: Solanum lycopersicum (tomato); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 130179.46

構造登録者

Contreras, M.P.,Pai, H.,Muniyandi, S.,Toghani, A.,Lawson, D.M.,Tumtas, Y.,Duggan, C.,Yuen, E.L.H.,Stevenson, C.E.M.,Harant, A.,Wu, C.H.,Bozkurt, T.O.,Kamoun, S.,Derevnina, L. (登録日: 2022-12-01, 公開日: 2022-12-21, 最終更新日: 2024-06-19)

主引用文献

Contreras, M.P.,Pai, H.,Selvaraj, M.,Toghani, A.,Lawson, D.M.,Tumtas, Y.,Duggan, C.,Yuen, E.L.H.,Stevenson, C.E.M.,Harant, A.,Maqbool, A.,Wu, C.H.,Bozkurt, T.O.,Kamoun, S.,Derevnina, L.
Resurrection of plant disease resistance proteins via helper NLR bioengineering.
Sci Adv, 9:eadg3861-eadg3861, 2023

PubMed Abstract: Parasites counteract host immunity by suppressing helper nucleotide binding and leucine-rich repeat (NLR) proteins that function as central nodes in immune receptor networks. Understanding the mechanisms of immunosuppression can lead to strategies for bioengineering disease resistance. Here, we show that a cyst nematode virulence effector binds and inhibits oligomerization of the helper NLR protein NRC2 by physically preventing intramolecular rearrangements required for activation. An amino acid polymorphism at the binding interface between NRC2 and the inhibitor is sufficient for this helper NLR to evade immune suppression, thereby restoring the activity of multiple disease resistance genes. This points to a potential strategy for resurrecting disease resistance in crop genomes.

PubMed: 37134163
DOI: 10.1126/sciadv.adg3861

実験手法

X-RAY DIFFRACTION (4.5 Å)