8BTK

Structure of the TRAP complex with the Sec translocon and a translating ribosome

これはPDB形式変換不可エントリーです。

8BTK の概要

• エントリーDOI: 10.2210/pdb8btk/pdb
• EMDBエントリー: 16232
• 分子名称: 28S rRNA, 60S ribosomal protein L7a, 60S ribosomal protein L9, ... (98 entities in total)
• 機能のキーワード: er targeting, protein translocation, er protein biogenesis, translocase
• 由来する生物種: Saccharomyces cerevisiae; 詳細
• タンパク質・核酸の鎖数: 92
• 化学式量合計: 4037976.00

構造登録者

Jaskolowski, M.,Jomaa, A.,Gamerdinger, M.,Shrestha, S.,Leibundgut, M.,Deuerling, E.,Ban, N. (登録日: 2022-11-29, 公開日: 2023-05-24, 最終更新日: 2024-04-24)

主引用文献

Jaskolowski, M.,Jomaa, A.,Gamerdinger, M.,Shrestha, S.,Leibundgut, M.,Deuerling, E.,Ban, N.
Molecular basis of the TRAP complex function in ER protein biogenesis.
Nat.Struct.Mol.Biol., 30:770-777, 2023

PubMed Abstract: The translocon-associated protein (TRAP) complex resides in the endoplasmic reticulum (ER) membrane and interacts with the Sec translocon and the ribosome to facilitate biogenesis of secretory and membrane proteins. TRAP plays a key role in the secretion of many hormones, including insulin. Here we reveal the molecular architecture of the mammalian TRAP complex and how it engages the translating ribosome associated with Sec61 translocon on the ER membrane. The TRAP complex is anchored to the ribosome via a long tether and its position is further stabilized by a finger-like loop. This positions a cradle-like lumenal domain of TRAP below the translocon for interactions with translocated nascent chains. Our structure-guided TRAP mutations in Caenorhabditis elegans lead to growth deficits associated with increased ER stress and defects in protein hormone secretion. These findings elucidate the molecular basis of the TRAP complex in the biogenesis and translocation of proteins at the ER.

PubMed: 37170030
DOI: 10.1038/s41594-023-00990-0

実験手法

ELECTRON MICROSCOPY (3.5 Å)