8BTG

Cryo-EM structure of the bacterial replication origin opening basal unwinding system

8BTG の概要

• エントリーDOI: 10.2210/pdb8btg/pdb
• EMDBエントリー: 16229
• 分子名称: Chromosomal replication initiator protein DnaA, DNA (5'-D(P*AP*CP*TP*TP*AP*TP*CP*CP*AP*CP*AP*AP*AP*TP*CP*CP*AP*CP*AP*GP*GP*CP*CP*C)-3'), DNA (41-MER), ... (5 entities in total)
• 機能のキーワード: dnaa, cryo-em, bus complex, replication initiation, replication
• 由来する生物種: Bacillus subtilis; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 380313.23

構造登録者

Pelliciari, S.,Bodet-Lefevre, S.,Murray, H.,Ilangovan, A. (登録日: 2022-11-28, 公開日: 2024-02-14, 最終更新日: 2025-07-09)

主引用文献

Pelliciari, S.,Bodet-Lefevre, S.,Fenyk, S.,Stevens, D.,Winterhalter, C.,Schramm, F.D.,Pintar, S.,Burnham, D.R.,Merces, G.,Richardson, T.T.,Tashiro, Y.,Hubbard, J.,Yardimci, H.,Ilangovan, A.,Murray, H.
The bacterial replication origin BUS promotes nucleobase capture.
Nat Commun, 14:8339-, 2023

PubMed Abstract: Genome duplication is essential for the proliferation of cellular life and this process is generally initiated by dedicated replication proteins at chromosome origins. In bacteria, DNA replication is initiated by the ubiquitous DnaA protein, which assembles into an oligomeric complex at the chromosome origin (oriC) that engages both double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) to promote DNA duplex opening. However, the mechanism of DnaA specifically opening a replication origin was unknown. Here we show that Bacillus subtilis DnaA assembles into a continuous oligomer at the site of DNA melting, extending from a dsDNA anchor to engage a single DNA strand. Within this complex, two nucleobases of each ssDNA binding motif (DnaA-trio) are captured within a dinucleotide binding pocket created by adjacent DnaA proteins. These results provide a molecular basis for DnaA specifically engaging the conserved sequence elements within the bacterial chromosome origin basal unwinding system (BUS).

PubMed: 38097584
DOI: 10.1093/nar/gkac1060

実験手法

ELECTRON MICROSCOPY (3.2 Å)