8BT1

YdaT transcription regulator (CII functional analog)

8BT1 の概要

• エントリーDOI: 10.2210/pdb8bt1/pdb
• 分子名称: YdaT_toxin domain-containing protein, GLYCEROL, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: cii functional analog, prophage cp-9339, dna binding protein
• 由来する生物種: Escherichia coli O157:H7
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 74367.46

構造登録者

Prolic-Kalinsek, M.,Loris, R. (登録日: 2022-11-27, 公開日: 2023-02-22, 最終更新日: 2024-06-19)

主引用文献

Prolic-Kalinsek, M.,Volkov, A.N.,Hadzi, S.,Van Dyck, J.,Bervoets, I.,Charlier, D.,Loris, R.
Structural basis of DNA binding by YdaT, a functional equivalent of the CII repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7.
Acta Crystallogr D Struct Biol, 79:245-258, 2023

PubMed Abstract: YdaT is a functional equivalent of the CII repressor in certain lambdoid phages and prophages. YdaT from the cryptic prophage CP-933P in the genome of Escherichia coli O157:H7 is functional as a DNA-binding protein and recognizes a 5'-TTGATTNAATCAA-3' inverted repeat. The DNA-binding domain is a helix-turn-helix (HTH)-containing POU domain and is followed by a long α-helix (α6) that forms an antiparallel four-helix bundle, creating a tetramer. The loop between helix α2 and the recognition helix α3 in the HTH motif is unusually long compared with typical HTH motifs, and is highly variable in sequence and length within the YdaT family. The POU domains have a large degree of freedom to move relative to the helix bundle in the free structure, but their orientation becomes fixed upon DNA binding.

PubMed: 36876434
DOI: 10.1107/S2059798323001249

実験手法

X-RAY DIFFRACTION (2.39788433003 Å)