8BQU

Molecular basis of ZP3/ZP1 heteropolymerization: crystal structure of a native vertebrate egg coat filament

8BQU の概要

• エントリーDOI: 10.2210/pdb8bqu/pdb
• 関連するPDBエントリー: 3NK4 5BUP 5OSQ 6TQK 8RKI
• 分子名称: Zona pellucida sperm-binding protein 3, Choriogenin H, beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: cell adhesion, fertilization, egg-sperm interaction, gamete recognition, sperm receptor, extracellular matrix, egg coat, zona pellucida, vitelline envelope, fish chorion, glycoprotein, n-glycan, structural protein, zp module, zp-n domain, zp_c domain, trefoil domain, medaka, japanese rice fish
• 由来する生物種: Oryzias latipes (Japanese medaka); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 72759.76

構造登録者

Bokhove, M.,de Sanctis, D.,Yasumasu, S.,Jovine, L. (登録日: 2022-11-21, 公開日: 2024-03-13, 最終更新日: 2026-03-04)

主引用文献

Nishio, S.,Emori, C.,Wiseman, B.,Fahrenkamp, D.,Dioguardi, E.,Zamora-Caballero, S.,Bokhove, M.,Han, L.,Stsiapanava, A.,Algarra, B.,Lu, Y.,Kodani, M.,Bainbridge, R.E.,Komondor, K.M.,Carlson, A.E.,Landreh, M.,de Sanctis, D.,Yasumasu, S.,Ikawa, M.,Jovine, L.
ZP2 cleavage blocks polyspermy by modulating the architecture of the egg coat.
Cell, 187:1440-1459.e24, 2024

PubMed Abstract: Following the fertilization of an egg by a single sperm, the egg coat or zona pellucida (ZP) hardens and polyspermy is irreversibly blocked. These events are associated with the cleavage of the N-terminal region (NTR) of glycoprotein ZP2, a major subunit of ZP filaments. ZP2 processing is thought to inactivate sperm binding to the ZP, but its molecular consequences and connection with ZP hardening are unknown. Biochemical and structural studies show that cleavage of ZP2 triggers its oligomerization. Moreover, the structure of a native vertebrate egg coat filament, combined with AlphaFold predictions of human ZP polymers, reveals that two protofilaments consisting of type I (ZP3) and type II (ZP1/ZP2/ZP4) components interlock into a left-handed double helix from which the NTRs of type II subunits protrude. Together, these data suggest that oligomerization of cleaved ZP2 NTRs extensively cross-links ZP filaments, rigidifying the egg coat and making it physically impenetrable to sperm.

PubMed: 38490181
DOI: 10.1016/j.cell.2024.02.013

実験手法

X-RAY DIFFRACTION (2.7 Å)