8BQS

Cryo-EM structure of the I-II-III2-IV2 respiratory supercomplex from Tetrahymena thermophila

これはPDB形式変換不可エントリーです。

8BQS の概要

• エントリーDOI: 10.2210/pdb8bqs/pdb
• EMDBエントリー: 16184
• 分子名称: Lipid-A-disaccharide synthase, Transmembrane protein, putative, Cytochrome C oxidase subunit Vb protein, ... (173 entities in total)
• 機能のキーワード: ciliate, mitochondrial, supercomplex, electron transport
• 由来する生物種: Tetrahymena thermophila SB210; 詳細
• タンパク質・核酸の鎖数: 214
• 化学式量合計: 6250466.44

構造登録者

Muhleip, A.,Kock Flygaard, R.,Baradaran, R.,Amunts, A. (登録日: 2022-11-21, 公開日: 2023-08-23, 最終更新日: 2025-02-19)

主引用文献

Muhleip, A.,Flygaard, R.K.,Baradaran, R.,Haapanen, O.,Gruhl, T.,Tobiasson, V.,Marechal, A.,Sharma, V.,Amunts, A.
Structural basis of mitochondrial membrane bending by the I-II-III 2 -IV 2 supercomplex.
Nature, 615:934-938, 2023

PubMed Abstract: Mitochondrial energy conversion requires an intricate architecture of the inner mitochondrial membrane. Here we show that a supercomplex containing all four respiratory chain components contributes to membrane curvature induction in ciliates. We report cryo-electron microscopy and cryo-tomography structures of the supercomplex that comprises 150 different proteins and 311 bound lipids, forming a stable 5.8-MDa assembly. Owing to subunit acquisition and extension, complex I associates with a complex IV dimer, generating a wedge-shaped gap that serves as a binding site for complex II. Together with a tilted complex III dimer association, it results in a curved membrane region. Using molecular dynamics simulations, we demonstrate that the divergent supercomplex actively contributes to the membrane curvature induction and tubulation of cristae. Our findings highlight how the evolution of protein subunits of respiratory complexes has led to the I-II-III-IV supercomplex that contributes to the shaping of the bioenergetic membrane, thereby enabling its functional specialization.

PubMed: 36949187
DOI: 10.1038/s41586-023-05817-y

実験手法

ELECTRON MICROSCOPY (2.9 Å)