8BOR

Photosensory module from DrBphP without PHY tongue

8BOR の概要

• エントリーDOI: 10.2210/pdb8bor/pdb
• 分子名称: Bacteriophytochrome, 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid (3 entities in total)
• 機能のキーワード: kinase, photosensor, transferase, phytochrome
• 由来する生物種: Deinococcus radiodurans R1; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 215740.62

構造登録者

Kurttila, M.,Takala, H.,Ihalainen, J.A. (登録日: 2022-11-15, 公開日: 2023-06-28, 最終更新日: 2024-11-13)

主引用文献

Kurttila, M.,Rumfeldt, J.,Takala, H.,Ihalainen, J.A.
The interconnecting hairpin extension "arm": An essential allosteric element of phytochrome activity.
Structure, 31:1100-, 2023

PubMed Abstract: In red-light sensing phytochromes, isomerization of the bilin chromophore triggers structural and dynamic changes across multiple domains, ultimately leading to control of the output module (OPM) activity. In between, a hairpin structure, "arm", extends from an interconnecting domain to the chromophore region. Here, by removing this protein segment in a bacteriophytochrome from Deinococcus radiodurans (DrBphP), we show that the arm is crucial for signal transduction. Crystallographic, spectroscopic, and biochemical data indicate that this variant maintains the properties of DrBphP in the resting state. Spectroscopic data also reveal that the armless systems maintain the ability to respond to light. However, there is no subsequent regulation of OPM activity without the arms. Thermal denaturation reveals that the arms stabilize the DrBphP structure. Our results underline the importance of the structurally flexible interconnecting hairpin extensions and describe their central role in the allosteric coupling of phytochromes.

PubMed: 37392739
DOI: 10.1016/j.str.2023.06.007

実験手法

X-RAY DIFFRACTION (2.3 Å)