8BN3

Yeast 80S, ES7s delta, eIF5A, Stm1 containing

これはPDB形式変換不可エントリーです。

8BN3 の概要

• エントリーDOI: 10.2210/pdb8bn3/pdb
• EMDBエントリー: 16127
• 分子名称: 60S ribosomal protein L1-A, 60S ribosomal protein L41-A, 5S ribosomal RNA, ... (87 entities in total)
• 機能のキーワード: eif5a, stm1, rpl41a, delta es7s, ribosome
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 81
• 化学式量合計: 2979329.97

構造登録者

Dimitrova-Paternoga, L.,Paternoga, H.,Wilson, D.N. (登録日: 2022-11-12, 公開日: 2024-01-10, 最終更新日: 2024-10-16)

主引用文献

Rauscher, R.,Eggers, C.,Dimitrova-Paternoga, L.,Shankar, V.,Rosina, A.,Cristodero, M.,Paternoga, H.,Wilson, D.N.,Leidel, S.A.,Polacek, N.
Evolving precision: rRNA expansion segment 7S modulates translation velocity and accuracy in eukaryal ribosomes.
Nucleic Acids Res., 52:4021-4036, 2024

PubMed Abstract: Ribosome-enhanced translational miscoding of the genetic code causes protein dysfunction and loss of cellular fitness. During evolution, open reading frame length increased, necessitating mechanisms for enhanced translation fidelity. Indeed, eukaryal ribosomes are more accurate than bacterial counterparts, despite their virtually identical, conserved active centers. During the evolution of eukaryotic organisms ribosome expansions at the rRNA and protein level occurred, which potentially increases the options for translation regulation and cotranslational events. Here we tested the hypothesis that ribosomal RNA expansions can modulate the core function of the ribosome, faithful protein synthesis. We demonstrate that a short expansion segment present in all eukaryotes' small subunit, ES7S, is crucial for accurate protein synthesis as its presence adjusts codon-specific velocities and guarantees high levels of cognate tRNA selection. Deletion of ES7S in yeast enhances mistranslation and causes protein destabilization and aggregation, dramatically reducing cellular fitness. Removal of ES7S did not alter ribosome architecture but altered the structural dynamics of inter-subunit bridges thus affecting A-tRNA selection. Exchanging the yeast ES7S sequence with the human ES7S increases accuracy whereas shortening causes the opposite effect. Our study demonstrates that ES7S provided eukaryal ribosomes with higher accuracy without perturbing the structurally conserved decoding center.

PubMed: 38324474
DOI: 10.1093/nar/gkae067

実験手法

ELECTRON MICROSCOPY (2.4 Å)