8BK2

X-ray structure of meningococcal factor H binding protein variant 2 in complex with a specific and bactericidal human monoclonal antibody 1B1

8BK2 の概要

• エントリーDOI: 10.2210/pdb8bk2/pdb
• 分子名称: Factor H-binding protein, Fab Heavy chain, Fab light Chains, ... (10 entities in total)
• 機能のキーワード: meningococcus, antigen, human monoclonal, fhbp, bactericidal, factor h displacement, vaccine., protein binding
• 由来する生物種: Neisseria meningitidis serogroup B; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 228071.47

構造登録者

Veggi, D.,Bottomley, J.M. (登録日: 2022-11-08, 公開日: 2023-11-22, 最終更新日: 2024-11-20)

主引用文献

Veggi, D.,Chesterman, C.C.,Santini, L.,Huang, Y.,Pacchiani, N.,Sierra, J.,Chen, L.,Laliberte, J.,Bianchi, F.,Cozzi, R.,Frigimelica, E.,Maione, D.,Finco, O.,Bottomley, M.J.
Bactericidal human monoclonal antibody 1B1 shows specificity for meningococcal factor H binding protein variant 2 and displaces human factor H.
Faseb Bioadv, 6:235-248, 2024

PubMed Abstract: Thousands of disease cases and hundreds of deaths occur globally each year due to invasive meningococcal disease. serogroup B (MenB) is the leading cause of such disease in developed countries. Two vaccines, 4CMenB and MenB-fHbp, that protect against MenB are available and include one or two forms respectively of factor H binding protein (fHbp), a key protective antigen. Studies of circulating meningococci have identified over 1380 different fHbp amino acid sequences, which form three immunologically distinct clusters, termed variants 1, 2, and 3. Neither of the current vaccines contains a variant 2 antigen, which is less well characterized than fHbp variants 1 and 3. We characterized the interaction of fHbp variant 2 with humAb 1B1 using biochemical methods and live meningococcal assays. Further, we determined the crystal structure of the complex at 2.4 Å resolution, clearly revealing the epitope and providing the first detailed report of an antibody with distinct specificity for fHbp variant 2. Extensive mutagenesis and binding studies elucidated key hotspots in the interface. This combination of structural and functional studies provides a molecular explanation for the bactericidal potency and specificity of humAb 1B1 for fHbp variant 2. Our studies, focused on fHbp variant 2, expand the understanding of this previously under characterized group of the vast family of variants of fHbp, a virulence factor present on all meningococci. Moreover, the definition of a protective conformational epitope on fHbp variant 2 may support the design and development of novel variant 2-containing MenB vaccines affording greater breadth of protection.

PubMed: 39114449
DOI: 10.1096/fba.2023-00077

実験手法

X-RAY DIFFRACTION (2.41 Å)