8BJ6

Crystal structure of YopR

8BJ6 の概要

• エントリーDOI: 10.2210/pdb8bj6/pdb
• 分子名称: SPbeta prophage-derived uncharacterized protein YopR (2 entities in total)
• 機能のキーワード: yopr, phage-repressor, recombinase, dna binding protein
• 由来する生物種: Bacillus subtilis subsp. subtilis str. 168
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 112303.55

構造登録者

Gallego del Sol, F.,Marina, A. (登録日: 2022-11-03, 公開日: 2023-11-15, 最終更新日: 2024-11-20)

主引用文献

Brady, A.,Cabello-Yeves, E.,Gallego Del Sol, F.,Chmielowska, C.,Mancheno-Bonillo, J.,Zamora-Caballero, S.,Omer, S.B.,Torres-Puente, M.,Eldar, A.,Quiles-Puchalt, N.,Marina, A.,Penades, J.R.
Characterization of a unique repression system present in arbitrium phages of the SPbeta family.
Cell Host Microbe, 31:2023-2037.e8, 2023

PubMed Abstract: Arbitrium-coding phages use peptides to communicate and coordinate the decision between lysis and lysogeny. However, the mechanism by which these phages establish lysogeny remains unknown. Here, focusing on the SPbeta phage family's model phages phi3T and SPβ, we report that a six-gene operon called the "SPbeta phages repressor operon" (sro) expresses not one but two master repressors, SroE and SroF, the latter of which folds like a classical phage integrase. To promote lysogeny, these repressors bind to multiple sites in the phage genome. SroD serves as an auxiliary repressor that, with SroEF, forms the repression module necessary for lysogeny establishment and maintenance. Additionally, the proteins SroABC within the operon are proposed to constitute the transducer module, connecting the arbitrium communication system to the activity of the repression module. Overall, this research sheds light on the intricate and specialized repression system employed by arbitrium SPβ-like phages in making lysis-lysogeny decisions.

PubMed: 38035880
DOI: 10.1016/j.chom.2023.11.003

実験手法

X-RAY DIFFRACTION (2.6 Å)