8BGF

NMR solution structure of the N-terminal RRM and flanking linker regions of Polypyrimidine tract binding protein 1 using the CYANA CONSENSUS method.

8BGF の概要

• エントリーDOI: 10.2210/pdb8bgf/pdb
• 関連するPDBエントリー: 2N3O
• NMR情報: BMRB: 34766
• 分子名称: Polypyrimidine tract-binding protein 1 (1 entity in total)
• 機能のキーワード: rna binding protein alternative splicing repressor ires trans-acting factor rna recognition motif dynamic structure, rna binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13375.17

構造登録者

Damberger, F.D.,Beusch, I.,Allain, F.H.-T. (登録日: 2022-10-27, 公開日: 2023-11-08, 最終更新日: 2024-10-02)

主引用文献

Damberger, F.F.,Krepl, M.,Arora, R.,Beusch, I.,Maris, C.,Dorn, G.,Sponer, J.,Ravindranathan, S.,Allain, F.H.
N-terminal domain of polypyrimidine-tract binding protein is a dynamic folding platform for adaptive RNA recognition.
Nucleic Acids Res., 52:10683-10704, 2024

PubMed Abstract: The N-terminal RNA recognition motif domain (RRM1) of polypyrimidine tract binding protein (PTB) forms an additional C-terminal helix α3, which docks to one edge of the β-sheet upon binding to a stem-loop RNA containing a UCUUU pentaloop. Importantly, α3 does not contact the RNA. The α3 helix therefore represents an allosteric means to regulate the conformation of adjacent domains in PTB upon binding structured RNAs. Here we investigate the process of dynamic adaptation by stem-loop RNA and RRM1 using NMR and MD in order to obtain mechanistic insights on how this allostery is achieved. Relaxation data and NMR structure determination of the free protein show that α3 is partially ordered and interacts with the domain transiently. Stem-loop RNA binding quenches fast time scale dynamics and α3 becomes ordered, however microsecond dynamics at the protein-RNA interface is observed. MD shows how RRM1 binding to the stem-loop RNA is coupled to the stabilization of the C-terminal helix and helps to transduce differences in RNA loop sequence into changes in α3 length and order. IRES assays of full length PTB and a mutant with altered dynamics in the α3 region show that this dynamic allostery influences PTB function in cultured HEK293T cells.

PubMed: 39180402
DOI: 10.1093/nar/gkae713

実験手法

SOLUTION NMR