8BFY

ABC transporter binding protein CebE from Streptomyces scabiei in complex with cellotriose

8BFY の概要

• エントリーDOI: 10.2210/pdb8bfy/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900021
• 分子名称: Putative secreted cellobiose-binding (Transport system associated), beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: abc transporter, maltose binding protein, carbohydrate
• 由来する生物種: Streptomyces scabiei
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46449.52

構造登録者

Rigali, S.,Jourdan, S.,Kerff, F. (登録日: 2022-10-27, 公開日: 2023-02-01, 最終更新日: 2024-11-13)

主引用文献

Kerff, F.,Jourdan, S.,Francis, I.M.,Deflandre, B.,Ribeiro Monteiro, S.,Stulanovic, N.,Loria, R.,Rigali, S.
Common scab disease: structural basis of elicitor recognition in pathogenic Streptomyces species.
Microbiol Spectr, 11:e0197523-e0197523, 2023

PubMed Abstract: Common scab is a disease caused by a few species that affects important root and tuber crops including potato, beet, radish, and parsnip, resulting in major economic losses worldwide. In this work, we unveiled the molecular basis of host recognition by these pathogens by solving the structure of the sugar-binding protein CebE of in complex with cellotriose, the main elicitor of the pathogenic lifestyle of these bacteria. We further revealed that the signaling pathway from CebE-mediated transport of cellotriose is conserved in all pathogenic species except which causes soft rot disease in sweet potatoes. Our work also provides the structural basis of the uptake of cellobiose and cellotriose in saprophytic species, the first step activating the expression of the enzymatic system degrading the most abundant polysaccharide on earth, cellulose.

PubMed: 37791952
DOI: 10.1128/spectrum.01975-23

実験手法

X-RAY DIFFRACTION (1.55 Å)