8BAD
Tpp80Aa1
Summary for 8BAD
Entry DOI | 10.2210/pdb8bad/pdb |
Descriptor | Binary toxin A-like protein, CALCIUM ION, 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (5 entities in total) |
Functional Keywords | bacillus thuriengiensis, mosquito, mosquitocidal, toxin, pesticidal protein |
Biological source | Bacillus thuringiensis |
Total number of polymer chains | 2 |
Total formula weight | 86327.58 |
Authors | Best, H.L.,Williamson, L.J.,Rizkallah, P.J.,Berry, C.,Lloyd-Evans, E. (deposition date: 2022-10-11, release date: 2022-12-14, Last modification date: 2024-01-31) |
Primary citation | Best, H.L.,Williamson, L.J.,Lipka-Lloyd, M.,Waller-Evans, H.,Lloyd-Evans, E.,Rizkallah, P.J.,Berry, C. The Crystal Structure of Bacillus thuringiensis Tpp80Aa1 and Its Interaction with Galactose-Containing Glycolipids. Toxins, 14:-, 2022 Cited by PubMed Abstract: Tpp80Aa1 from is a Toxin_10 family protein (Tpp) with reported action against mosquitoes. Here, we demonstrate an expanded target range, showing Tpp80Aa1 is also active against the larvae of and mosquitoes. We report the first crystal structure of Tpp80Aa1 at a resolution of 1.8 Å, which shows Tpp80Aa1 consists of two domains: an N-terminal β-trefoil domain resembling a ricin B lectin and a C-terminal putative pore-forming domain sharing structural similarity with the aerolysin family. Similar to other Tpp family members, we observe Tpp80Aa1 binds to the mosquito midgut, specifically the posterior midgut and the gastric caecum. We also identify that Tpp80Aa1 can interact with galactose-containing glycolipids and galactose, and this interaction is critical for exerting full insecticidal action against mosquito target cell lines. PubMed: 36548760DOI: 10.3390/toxins14120863 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.81 Å) |
Structure validation
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