8B6U
Mpf2Ba1 monomer
Summary for 8B6U
Entry DOI | 10.2210/pdb8b6u/pdb |
EMDB information | 15882 15883 |
Descriptor | Mpf2Ba1, MAGNESIUM ION (3 entities in total) |
Functional Keywords | monomer, pore-forming protein, macpf, insecticidal, toxin |
Biological source | Pseudomonas monteilii |
Total number of polymer chains | 1 |
Total formula weight | 53217.59 |
Authors | Marini, G.,Poland, B.,Leininger, C.,Lukoyanova, N.,Spielbauer, D.,Barry, J.,Altier, D.,Lum, A.,Scolaro, E.,Perez-Ortega, C.,Yalpani, N.,Sandahl, G.,Mabry, T.,Klever, J.,Nowatzki, T.,Zhao, J.Z.,Sethi, A.,Kassa, A.,Crane, V.,Lu, A.,Nelson, M.E.,Eswar, N.,Topf, M.,Saibil, H.R. (deposition date: 2022-09-27, release date: 2023-05-31, Last modification date: 2024-02-07) |
Primary citation | Marini, G.,Poland, B.,Leininger, C.,Lukoyanova, N.,Spielbauer, D.,Barry, J.K.,Altier, D.,Lum, A.,Scolaro, E.,Ortega, C.P.,Yalpani, N.,Sandahl, G.,Mabry, T.,Klever, J.,Nowatzki, T.,Zhao, J.Z.,Sethi, A.,Kassa, A.,Crane, V.,Lu, A.L.,Nelson, M.E.,Eswar, N.,Topf, M.,Saibil, H.R. Structural journey of an insecticidal protein against western corn rootworm. Nat Commun, 14:4171-4171, 2023 Cited by PubMed Abstract: The broad adoption of transgenic crops has revolutionized agriculture. However, resistance to insecticidal proteins by agricultural pests poses a continuous challenge to maintaining crop productivity and new proteins are urgently needed to replace those utilized for existing transgenic traits. We identified an insecticidal membrane attack complex/perforin (MACPF) protein, Mpf2Ba1, with strong activity against the devastating coleopteran pest western corn rootworm (WCR) and a novel site of action. Using an integrative structural biology approach, we determined monomeric, pre-pore and pore structures, revealing changes between structural states at high resolution. We discovered an assembly inhibition mechanism, a molecular switch that activates pre-pore oligomerization upon gut fluid incubation and solved the highest resolution MACPF pore structure to-date. Our findings demonstrate not only the utility of Mpf2Ba1 in the development of biotechnology solutions for protecting maize from WCR to promote food security, but also uncover previously unknown mechanistic principles of bacterial MACPF assembly. PubMed: 37443175DOI: 10.1038/s41467-023-39891-7 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.134 Å) |
Structure validation
Download full validation report