8B6U

Mpf2Ba1 monomer

8B6U の概要

• エントリーDOI: 10.2210/pdb8b6u/pdb
• EMDBエントリー: 15882 15883
• 分子名称: Mpf2Ba1, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: monomer, pore-forming protein, macpf, insecticidal, toxin
• 由来する生物種: Pseudomonas monteilii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53217.59

構造登録者

Marini, G.,Poland, B.,Leininger, C.,Lukoyanova, N.,Spielbauer, D.,Barry, J.,Altier, D.,Lum, A.,Scolaro, E.,Perez-Ortega, C.,Yalpani, N.,Sandahl, G.,Mabry, T.,Klever, J.,Nowatzki, T.,Zhao, J.Z.,Sethi, A.,Kassa, A.,Crane, V.,Lu, A.,Nelson, M.E.,Eswar, N.,Topf, M.,Saibil, H.R. (登録日: 2022-09-27, 公開日: 2023-05-31, 最終更新日: 2024-02-07)

主引用文献

Marini, G.,Poland, B.,Leininger, C.,Lukoyanova, N.,Spielbauer, D.,Barry, J.K.,Altier, D.,Lum, A.,Scolaro, E.,Ortega, C.P.,Yalpani, N.,Sandahl, G.,Mabry, T.,Klever, J.,Nowatzki, T.,Zhao, J.Z.,Sethi, A.,Kassa, A.,Crane, V.,Lu, A.L.,Nelson, M.E.,Eswar, N.,Topf, M.,Saibil, H.R.
Structural journey of an insecticidal protein against western corn rootworm.
Nat Commun, 14:4171-4171, 2023

PubMed Abstract: The broad adoption of transgenic crops has revolutionized agriculture. However, resistance to insecticidal proteins by agricultural pests poses a continuous challenge to maintaining crop productivity and new proteins are urgently needed to replace those utilized for existing transgenic traits. We identified an insecticidal membrane attack complex/perforin (MACPF) protein, Mpf2Ba1, with strong activity against the devastating coleopteran pest western corn rootworm (WCR) and a novel site of action. Using an integrative structural biology approach, we determined monomeric, pre-pore and pore structures, revealing changes between structural states at high resolution. We discovered an assembly inhibition mechanism, a molecular switch that activates pre-pore oligomerization upon gut fluid incubation and solved the highest resolution MACPF pore structure to-date. Our findings demonstrate not only the utility of Mpf2Ba1 in the development of biotechnology solutions for protecting maize from WCR to promote food security, but also uncover previously unknown mechanistic principles of bacterial MACPF assembly.

PubMed: 37443175
DOI: 10.1038/s41467-023-39891-7

実験手法

X-RAY DIFFRACTION (2.134 Å)