8B65
Structure of rsCherry crystallized in anaerobic conditions
Summary for 8B65
| Entry DOI | 10.2210/pdb8b65/pdb |
| Descriptor | rsCherry, DI(HYDROXYETHYL)ETHER, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (6 entities in total) |
| Functional Keywords | rscherry, red fluorescent protein, chemical modifications, fluorescent protein |
| Biological source | Discosoma sp. |
| Total number of polymer chains | 1 |
| Total formula weight | 31227.01 |
| Authors | Bui, T.Y.H.,Van Meervelt, L. (deposition date: 2022-09-26, release date: 2023-04-05, Last modification date: 2024-02-07) |
| Primary citation | Bui, T.Y.H.,De Zitter, E.,Moeyaert, B.,Pecqueur, L.,Srinivasu, B.Y.,Economou, A.,Fontecave, M.,Van Meervelt, L.,Dedecker, P.,Pedre, B. Oxygen-induced chromophore degradation in the photoswitchable red fluorescent protein rsCherry. Int.J.Biol.Macromol., 239:124179-124179, 2023 Cited by PubMed Abstract: Reversibly switchable monomeric Cherry (rsCherry) is a photoswitchable variant of the red fluorescent protein mCherry. We report that this protein gradually and irreversibly loses its red fluorescence in the dark over a period of months at 4 °C and a few days at 37 °C. We also find that its ancestor, mCherry, undergoes a similar fluorescence loss but at a slower rate. X-ray crystallography and mass spectrometry reveal that this is caused by the cleavage of the p-hydroxyphenyl ring from the chromophore and the formation of two novel types of cyclic structures at the remaining chromophore moiety. Overall, our work sheds light on a new process occurring within fluorescent proteins, further adding to the chemical diversity and versatility of these molecules. PubMed: 36972828DOI: 10.1016/j.ijbiomac.2023.124179 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.55 Å) |
Structure validation
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