8B64

Cryo-EM structure of RC-LH1-PufX photosynthetic core complex from Rba. capsulatus

8B64 の概要

• エントリーDOI: 10.2210/pdb8b64/pdb
• EMDBエントリー: 15862
• 分子名称: Light-harvesting protein B-870 alpha chain, UBIQUINONE-10, 1,2-Distearoyl-sn-glycerophosphoethanolamine, ... (12 entities in total)
• 機能のキーワード: pufx, core complex, rc-lh1, rba capsulatus, photosynthetic core, photosynthesis
• 由来する生物種: Rhodobacter capsulatus; 詳細
• タンパク質・核酸の鎖数: 34
• 化学式量合計: 341138.17

構造登録者

Bracun, L.,Yamagata, A.,Shirouzu, M.,Liu, L.N. (登録日: 2022-09-26, 公開日: 2023-02-01, 最終更新日: 2024-07-24)

主引用文献

Bracun, L.,Yamagata, A.,Christianson, B.M.,Shirouzu, M.,Liu, L.N.
Cryo-EM structure of a monomeric RC-LH1-PufX supercomplex with high-carotenoid content from Rhodobacter capsulatus.
Structure, 31:318-, 2023

PubMed Abstract: In purple photosynthetic bacteria, the photochemical reaction center (RC) and light-harvesting complex 1 (LH1) assemble to form monomeric or dimeric RC-LH1 membrane complexes, essential for bacterial photosynthesis. Here, we report a 2.59-Å resolution cryoelectron microscopy (cryo-EM) structure of the RC-LH1 supercomplex from Rhodobacter capsulatus. We show that Rba. capsulatus RC-LH1 complexes are exclusively monomers in which the RC is surrounded by a 15-subunit LH1 ring. Incorporation of a transmembrane polypeptide PufX leads to a large opening within the LH1 ring. Each LH1 subunit associates two carotenoids and two bacteriochlorophylls, which is similar to Rba. sphaeroides RC-LH1 but more than one carotenoid per LH1 in Rba. veldkampii RC-LH1 monomer. Collectively, the unique Rba. capsulatus RC-LH1-PufX represents an intermediate structure between Rba. sphaeroides and Rba. veldkampii RC-LH1-PufX. Comparison of PufX from the three Rhodobacter species indicates the important residues involved in dimerization of RC-LH1.

PubMed: 36738736
DOI: 10.1016/j.str.2023.01.006

実験手法

ELECTRON MICROSCOPY (2.589 Å)