8B2L

Cryo-EM structure of the plant 80S ribosome

これはPDB形式変換不可エントリーです。

8B2L の概要

• エントリーDOI: 10.2210/pdb8b2l/pdb
• EMDBエントリー: 15806
• 分子名称: 40S ribosomal protein S4, 40S ribosomal protein S24, 40S ribosomal protein S25, ... (88 entities in total)
• 機能のキーワード: plant, 80s, rrna modifications, ribosome
• 由来する生物種: Nicotiana tabacum (common tobacco); 詳細
• タンパク質・核酸の鎖数: 83
• 化学式量合計: 3262704.91

構造登録者

Smirnova, J.,Loerke, J.,Kleinau, G.,Schmidt, A.,Buerger, J.,Meyer, E.H.,Mielke, T.,Scheerer, P.,Bock, R.,Spahn, C.M.T.,Zoschke, R. (登録日: 2022-09-14, 公開日: 2023-08-23, 最終更新日: 2024-04-24)

主引用文献

Smirnova, J.,Loerke, J.,Kleinau, G.,Schmidt, A.,Burger, J.,Meyer, E.H.,Mielke, T.,Scheerer, P.,Bock, R.,Spahn, C.M.T.,Zoschke, R.
Structure of the actively translating plant 80S ribosome at 2.2 angstrom resolution.
Nat.Plants, 9:987-1000, 2023

PubMed Abstract: In plant cells, translation occurs in three compartments: the cytosol, the plastids and the mitochondria. While the structures of the (prokaryotic-type) ribosomes in plastids and mitochondria are well characterized, high-resolution structures of the eukaryotic 80S ribosomes in the cytosol have been lacking. Here the structure of translating tobacco (Nicotiana tabacum) 80S ribosomes was solved by cryo-electron microscopy with a global resolution of 2.2 Å. The ribosome structure includes two tRNAs, decoded mRNA and the nascent peptide chain, thus providing insights into the molecular underpinnings of the cytosolic translation process in plants. The map displays conserved and plant-specific rRNA modifications and the positions of numerous ionic cofactors, and it uncovers the role of monovalent ions in the decoding centre. The model of the plant 80S ribosome enables broad phylogenetic comparisons that reveal commonalities and differences in the ribosomes of plants and those of other eukaryotes, thus putting our knowledge about eukaryotic translation on a firmer footing.

PubMed: 37156858
DOI: 10.1038/s41477-023-01407-y

実験手法

ELECTRON MICROSCOPY (2.2 Å)