8B0X

Translating 70S ribosome in the unrotated state (P and E, tRNAs)

これはPDB形式変換不可エントリーです。

8B0X の概要

• エントリーDOI: 10.2210/pdb8b0x/pdb
• EMDBエントリー: 15793 15794 15795 15796 15797
• 分子名称: 50S ribosomal protein L33, 30S ribosomal protein S6, 30S ribosomal protein S7, ... (57 entities in total)
• 機能のキーワード: 70s, bacterial, translation, high-resolution, ribosome
• 由来する生物種: Escherichia coli B; 詳細
• タンパク質・核酸の鎖数: 53
• 化学式量合計: 2185230.16

構造登録者

Fromm, S.A.,O'Connor, K.M.,Purdy, M.,Bhatt, P.R.,Loughran, G.,Atkins, J.F.,Jomaa, A.,Mattei, S. (登録日: 2022-09-08, 公開日: 2022-11-30, 最終更新日: 2026-04-01)

主引用文献

Fromm, S.A.,O'Connor, K.M.,Purdy, M.,Bhatt, P.R.,Loughran, G.,Atkins, J.F.,Jomaa, A.,Mattei, S.
The translating bacterial ribosome at 1.55 angstrom resolution generated by cryo-EM imaging services.
Nat Commun, 14:1095-1095, 2023

PubMed Abstract: Our understanding of protein synthesis has been conceptualised around the structure and function of the bacterial ribosome. This complex macromolecular machine is the target of important antimicrobial drugs, an integral line of defence against infectious diseases. Here, we describe how open access to cryo-electron microscopy facilities combined with bespoke user support enabled structural determination of the translating ribosome from Escherichia coli at 1.55 Å resolution. The obtained structures allow for direct determination of the rRNA sequence to identify ribosome polymorphism sites in the E. coli strain used in this study and enable interpretation of the ribosomal active and peripheral sites at unprecedented resolution. This includes scarcely populated chimeric hybrid states of the ribosome engaged in several tRNA translocation steps resolved at ~2 Å resolution. The current map not only improves our understanding of protein synthesis but also allows for more precise structure-based drug design of antibiotics to tackle rising bacterial resistance.

PubMed: 36841832
DOI: 10.1038/s41467-023-36742-3

実験手法

ELECTRON MICROSCOPY (1.55 Å)