8AXT

Sialidases and Fucosidases of Akkermansia muciniphila are key for rapid growth on colonic mucin and nutrient sharing amongst mucin-associated human gut microbiota

8AXT の概要

• エントリーDOI: 10.2210/pdb8axt/pdb
• 分子名称: Sialidase domain-containing protein, CALCIUM ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: sialidase, neuraminidase, hydrolase
• 由来する生物種: Akkermansia muciniphila
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 130556.24

構造登録者

Sakanaka, H.,Nielsen, T.S.,Pichler, M.J.,Nordberg Karlsson, E.,Abou Hachem, M.,Morth, J.P. (登録日: 2022-08-31, 公開日: 2023-03-01, 最終更新日: 2024-05-01)

主引用文献

Shuoker, B.,Pichler, M.J.,Jin, C.,Sakanaka, H.,Wu, H.,Gascuena, A.M.,Liu, J.,Nielsen, T.S.,Holgersson, J.,Nordberg Karlsson, E.,Juge, N.,Meier, S.,Morth, J.P.,Karlsson, N.G.,Abou Hachem, M.
Sialidases and fucosidases of Akkermansia muciniphila are crucial for growth on mucin and nutrient sharing with mucus-associated gut bacteria.
Nat Commun, 14:1833-1833, 2023

PubMed Abstract: The mucolytic human gut microbiota specialist Akkermansia muciniphila is proposed to boost mucin-secretion by the host, thereby being a key player in mucus turnover. Mucin glycan utilization requires the removal of protective caps, notably fucose and sialic acid, but the enzymatic details of this process remain largely unknown. Here, we describe the specificities of ten A. muciniphila glycoside hydrolases, which collectively remove all known sialyl and fucosyl mucin caps including those on double-sulfated epitopes. Structural analyses revealed an unprecedented fucosidase modular arrangement and explained the sialyl T-antigen specificity of a sialidase of a previously unknown family. Cell-attached sialidases and fucosidases displayed mucin-binding and their inhibition abolished growth of A. muciniphila on mucin. Remarkably, neither the sialic acid nor fucose contributed to A. muciniphila growth, but instead promoted butyrate production by co-cultured Clostridia. This study brings unprecedented mechanistic insight into the initiation of mucin O-glycan degradation by A. muciniphila and nutrient sharing between mucus-associated bacteria.

PubMed: 37005422
DOI: 10.1038/s41467-023-37533-6

実験手法

X-RAY DIFFRACTION (1.59 Å)