8ASP

RCII/PSI complex, focused refinement of PSI

8ASP の概要

• エントリーDOI: 10.2210/pdb8asp/pdb
• EMDBエントリー: 15522 15618 15621
• 分子名称: Photosystem I P700 chlorophyll a apoprotein A1, Photosystem I reaction center subunit XI, Photosystem I reaction center subunit XII, ... (23 entities in total)
• 機能のキーワード: photosystem, assembly factor, membrane protein, photosynthesis
• 由来する生物種: Synechocystis sp. PCC 6803; 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 360600.32

構造登録者

Zhao, Z.,Vercellino, I.,Knoppova, J.,Sobotka, R.,Murray, J.W.,Nixon, P.J.,Sazanov, L.A.,Komenda, J. (登録日: 2022-08-20, 公開日: 2023-08-16, 最終更新日: 2024-10-16)

主引用文献

Zhao, Z.,Vercellino, I.,Knoppova, J.,Sobotka, R.,Murray, J.W.,Nixon, P.J.,Sazanov, L.A.,Komenda, J.
The Ycf48 accessory factor occupies the site of the oxygen-evolving manganese cluster during photosystem II biogenesis.
Nat Commun, 14:4681-4681, 2023

PubMed Abstract: Robust oxygenic photosynthesis requires a suite of accessory factors to ensure efficient assembly and repair of the oxygen-evolving photosystem two (PSII) complex. The highly conserved Ycf48 assembly factor binds to the newly synthesized D1 reaction center polypeptide and promotes the initial steps of PSII assembly, but its binding site is unclear. Here we use cryo-electron microscopy to determine the structure of a cyanobacterial PSII D1/D2 reaction center assembly complex with Ycf48 attached. Ycf48, a 7-bladed beta propeller, binds to the amino-acid residues of D1 that ultimately ligate the water-oxidising MnCaO cluster, thereby preventing the premature binding of Mn and Ca ions and protecting the site from damage. Interactions with D2 help explain how Ycf48 promotes assembly of the D1/D2 complex. Overall, our work provides valuable insights into the early stages of PSII assembly and the structural changes that create the binding site for the MnCaO cluster.

PubMed: 37542031
DOI: 10.1038/s41467-023-40388-6

実験手法

ELECTRON MICROSCOPY (2.9 Å)