8AP9

rotor of the Trypanosoma brucei mitochondrial ATP synthase dimer

8AP9 の概要

• エントリーDOI: 10.2210/pdb8ap9/pdb
• EMDBエントリー: 15562
• 分子名称: ATP synthase gamma subunit, ATP synthase, epsilon chain, putative, ATP synthase subunit epsilon, mitochondrial, ... (5 entities in total)
• 機能のキーワード: atp synthase, mitochondria, membrane protein
• 由来する生物種: Trypanosoma brucei brucei; 詳細
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 187824.92

構造登録者

Muehleip, A.,Gahura, O.,Zikova, A.,Amunts, A. (登録日: 2022-08-09, 公開日: 2022-10-26, 最終更新日: 2024-07-24)

主引用文献

Gahura, O.,Muhleip, A.,Hierro-Yap, C.,Panicucci, B.,Jain, M.,Hollaus, D.,Slapnickova, M.,Zikova, A.,Amunts, A.
An ancestral interaction module promotes oligomerization in divergent mitochondrial ATP synthases.
Nat Commun, 13:5989-5989, 2022

PubMed Abstract: Mitochondrial ATP synthase forms stable dimers arranged into oligomeric assemblies that generate the inner-membrane curvature essential for efficient energy conversion. Here, we report cryo-EM structures of the intact ATP synthase dimer from Trypanosoma brucei in ten different rotational states. The model consists of 25 subunits, including nine lineage-specific, as well as 36 lipids. The rotary mechanism is influenced by the divergent peripheral stalk, conferring a greater conformational flexibility. Proton transfer in the lumenal half-channel occurs via a chain of five ordered water molecules. The dimerization interface is formed by subunit-g that is critical for interactions but not for the catalytic activity. Although overall dimer architecture varies among eukaryotes, we find that subunit-g together with subunit-e form an ancestral oligomerization motif, which is shared between the trypanosomal and mammalian lineages. Therefore, our data defines the subunit-g/e module as a structural component determining ATP synthase oligomeric assemblies.

PubMed: 36220811
DOI: 10.1038/s41467-022-33588-z

実験手法

ELECTRON MICROSCOPY (3.7 Å)