8AMY

High-resolution crystal structure of the Mu8.1 conotoxin from Conus Mucronatus

8AMY の概要

• エントリーDOI: 10.2210/pdb8amy/pdb
• 分子名称: Mu8.1 conotoxin, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, 1-ETHOXY-2-(2-ETHOXYETHOXY)ETHANE, ... (8 entities in total)
• 機能のキーワード: conotoxin, toxin, antagonist, cav2.3 inhibitor, mu8.1
• 由来する生物種: Conus mucronatus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10902.58

構造登録者

Mueller, E.,Hackney, C.M.,Ellgaard, L.,Morth, J.P. (登録日: 2022-08-04, 公開日: 2023-08-23, 最終更新日: 2024-10-16)

主引用文献

Muller, E.,Hackney, C.M.,Ellgaard, L.,Morth, J.P.
High-resolution crystal structure of the Mu8.1 conotoxin from Conus mucronatus.
Acta Crystallogr.,Sect.F, 79:240-246, 2023

PubMed Abstract: Marine cone snails produce a wealth of peptide toxins (conotoxins) that bind their molecular targets with high selectivity and potency. Therefore, conotoxins constitute valuable biomolecular tools with a variety of biomedical purposes. The Mu8.1 conotoxin from Conus mucronatus is the founding member of the newly identified saposin-like conotoxin class of conotoxins and has been shown to target Cav2.3, a voltage-gated calcium channel. Two crystal structures have recently been determined of Mu8.1 at 2.3 and 2.1 Å resolution. Here, a high-resolution crystal structure of Mu8.1 was determined at 1.67 Å resolution in the high-symmetry space group I422. The asymmetric unit contained one molecule, with a symmetry-related molecule generating a dimer equivalent to that observed in the two previously determined structures. The high resolution allows a detailed atomic analysis of a water-filled cavity buried at the dimer interface, revealing a tightly coordinated network of waters that shield a lysine residue (Lys55) with a predicted unusually low side-chain pK value. These findings are discussed in terms of a potential functional role of Lys55 in target interaction.

PubMed: 37642664
DOI: 10.1107/S2053230X23007070

実験手法

X-RAY DIFFRACTION (1.67 Å)