8AMF

Cryo-EM structure of the RecA postsynaptic filament from S. pneumoniae

Summary for 8AMF

• Entry DOI: 10.2210/pdb8amf/pdb
• EMDB information: 15525
• Descriptor: Protein RecA, DNA, PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER, ... (4 entities in total)
• Functional Keywords: helical reconstruction, recombinase, streptococcus pneumoniae, double-stranded dna, recombination
• Biological source: Streptococcus pneumoniae; More
• Total number of polymer chains: 6
• Total formula weight: 176207.88

Authors

Perry, T.N.,Fronzes, R.,Polard, P.,Hertzog, M. (deposition date: 2022-08-03, release date: 2024-02-21, Last modification date: 2024-03-06)

Primary citation

Hertzog, M.,Perry, T.N.,Dupaigne, P.,Serres, S.,Morales, V.,Soulet, A.L.,Bell, J.C.,Margeat, E.,Kowalczykowski, S.C.,Le Cam, E.,Fronzes, R.,Polard, P.
Assembly mechanism and cryoEM structure of RecA recombination nucleofilaments from Streptococcus pneumoniae.
Nucleic Acids Res., 51:2800-2817, 2023

PubMed Abstract: RecA-mediated homologous recombination (HR) is a key mechanism for genome maintenance and plasticity in bacteria. It proceeds through RecA assembly into a dynamic filament on ssDNA, the presynaptic filament, which mediates DNA homology search and ordered DNA strand exchange. Here, we combined structural, single molecule and biochemical approaches to characterize the ATP-dependent assembly mechanism of the presynaptic filament of RecA from Streptococcus pneumoniae (SpRecA), in comparison to the Escherichia coli RecA (EcRecA) paradigm. EcRecA polymerization on ssDNA is assisted by the Single-Stranded DNA Binding (SSB) protein, which unwinds ssDNA secondary structures that block EcRecA nucleofilament growth. We report by direct microscopic analysis of SpRecA filamentation on ssDNA that neither of the two paralogous pneumococcal SSBs could assist the extension of SpRecA nucleopolymers. Instead, we found that the conserved RadA helicase promotes SpRecA nucleofilamentation in an ATP-dependent manner. This allowed us to solve the atomic structure of such a long native SpRecA nucleopolymer by cryoEM stabilized with ATPγS. It was found to be equivalent to the crystal structure of the EcRecA filament with a marked difference in how RecA mediates nucleotide orientation in the stretched ssDNA. Then, our results show that SpRecA and EcRecA HR activities are different, in correlation with their distinct ATP-dependent ssDNA binding modes.

PubMed: 36806960
DOI: 10.1093/nar/gkad080

Experimental method

ELECTRON MICROSCOPY (3.8 Å)